A DNA sample and a plasmid with the ampR and lacZ(for the enzyme beta-galactosidase) genes are treated with Pstl with the goal of making recombinant DNA molecules. the lacZ gene is placed in the cloning region so that it is disrupted by DNA inserts. Put the remaining steps for this process in the right order. NOT ALL statements will be used! The first step has already been given to you.
step options: -all the fragments have the sticky ends 5'-CTGCAG-3'
-cells containing the recombinant plasmid are grown in a medium with
ampicillin and x-gal(a galactose derivative)
-blue colonies that survive on the culture medium contain the
recombinant plasmid
-all the fragments have identical sticky ends: 5'-TGCA-3'
-colonies containing the recombinant plasmid can be removed from the
culture medium and grown
-DNA ligase joins the sticky ends of the DNA sample and the plasmid
-white colonies that survive on the culture medium are identified as
having the recombinant plasmid
step 1) DNA is treated with restriction enzyme
step 2)
step 3)
step 4)
step 5)
step 6)

Answers

Answer 1

Answer:

Explanation:

The main aim of this experiment is to correctly identify the sequential steps needed for making recombinant DNA molecules.

The first step is given as:

DNA is treated with restriction enzyme

The remaining steps in order are:

[tex]\text{-all the fragments have identical sticky ends: 5'-TGCA-3'}[/tex]

[tex]\text{-DNA ligase joins the sticky ends of the DNA sample and the plasmid}[/tex]

[tex]\text{-cells containing the recombinant plasmid are grown in a medium with}[/tex]

[tex]\text{ampicillin and x-gal(a galactose derivative)}[/tex]

[tex]\text{-white colonies that survive on the culture medium are identified as}[/tex]

[tex]\text{having the recombinant plasmid}[/tex]

[tex]\text{-colonies containing the recombinant plasmid can be removed from the}[/tex]

[tex]\text{culture medium and grown}[/tex]


Related Questions

When the number of molecules, atoms, or ions of a particular type (here we consider H ions) is in the hundreds of millions, we can confidently speak of concentrations and analyze an average behavior.

a. True
b. False

Answers

Answer:

True.

Explanation:

When the number of molecules, atoms or ions of any element is in the hundreds of millions, we can safely speak of concentrations and analyze a standard behavior, and can even specify the different dilutions used, if there are dilutions. This allows analyzes to be clearly understood by any individual. However, it is not possible to do this if the number of molecules, atoms and ions is very small.

Glve the orbital configuration of the following elements using the s, p, d, f type representation. (Answer format Is: 1se2 =
1s)
hellum, nitrogen, silicon
hellum nitrogen silicon

Answers

Answer:

Hellium (He): 1s2

Nitrogen (N): 1s2 2s2 2p6

Silicon (Si): 1s2 2s2 2p6 3s2 3p2

The orbital configuration of following elements is as follows:i) helium- 1 s² ii)nitrogen- 1 s² 2 s²  2 p³ iii)silicon-1 s² 2 s² 2 p⁶ 3 s² 3 p²

What are elements?

It is defined as a substance which cannot be broken down further into any other substance. Each element is made up of its own type of atom. Due to this reason all elements are different from one another.

Elements can be classified as metals and non-metals. Metals are shiny and conduct electricity and are all solids at room temperature except mercury. Non-metals do not conduct electricity and are mostly gases at room temperature except carbon and sulfur.

The number of protons in the nucleus is the defining property of an element and is related to the atomic number.All atoms with same atomic number are atoms of same element.

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Why is it necessary to run TLC in a closed container and to have the interior vapor saturated with the solvent? What error is introduced into the determination of an Rf value if the top is left off of the developing chamber?

Answers

Answer:

See explanation

Explanation:

Rf value is known as retention factor.The retention factor of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin(Harper College).

TLC is carried is out in a closed container and the interior is saturated with the solvent vapor in order to have a maximum resolution between components this prevents solvent from evaporating from the system.

Peradventure the solvent for the separation is partly or wholly  lost due to an open container, the Rf value would be lower than the expected value.

What type of reaction
the following chemical reaction?
2C2H4O2 → C4H6O3 + H2O

Answers

This type of chemical reaction is Decomposition

how can i balance Na + Ca2+ --> Ca + Na+

Answers

Answer:

[tex]2Na + Ca^{2+} \rightarrow Ca + 2Na^+[/tex]

Explanation:

Hello!

In this case, since this a reaction in which the oxidation state of the metals is changing, we need to apply a redox method for which:

[tex]Na\rightarrow Na^++e^-\\\\Ca^{2+}\rightarrow Ca+2e^-\\\\\\2Na\rightarrow 2Na^++2e^-\\\\Ca^{2+}\rightarrow Ca+2e^-[/tex]

Thus, the result is:

[tex]2Na + Ca^{2+} \rightarrow Ca + 2Na^+[/tex]

Regards!

What can she find out from her experiment?

A. If soaked bean seeds sprout faster

B. If dry bean seeds require more water

C. If dry bean seeds produce taller plants

D. If soaked bean seeds require more sunlight​

Answers

Answer:

B. If dry bean seeds require more water

I hope it is helpful..

(04.02 LC)
During photosynthesis, water and carbon dioxide become glucose and oxygen.
True
False

Answers

Answer:

true............,....

Answer:False

Explanation:

This statement is false. Correct statement: Photosynthesis produces glucose and oxygen. ... In this process, plants produce glucose and oxygen from carbon dioxide and water in the presence of sunlight. Photosynthesis converts light energy into chemical energy.

Given the following:

Measurement Reaction 1 (Mg + HCl)
Mass of HCl (g) 100.50
Mass of solid (g) 0.20
Total mass of reactants, m (g) (add the masses above) 100.70
Initial Temperature (°C) 22.5
Temperature furthest from initial temperature(°C) 31.3
∆T(°C) Subtract the two temperatures above) 8.8

Given Reaction 1: Mg(s) + 2HCl(aq) ⟶ MgCl2(aq) + H2(g)

What is the ∆H (kJ/mol)?

Answers

Answer:

∆H = - 450 kJ/mol

Explanation:

You can calculate the change in enthalpy provided we have the heat of solution (q). That would be the only approach to this problem. But this approach is only possible if we have a specific set-up; we would have to use a calorimeter. So then the reactants are taken as the system, and water as the surroundings. When calculating q, the heat of the solution, we take 4.18. Remember that heat is flowing from the reaction mixture, to the water, from the system to surroundings, so it's exothermic and ∆H = negative:

100. The compound Na FCN).NOJ is known as

Answers

Answer:

Na=sodium FCN=Cyanogon Flouride. NOJ =DEOXYNOJIRIMYCIN/MORANOLINE

How many electrons were transferred if the total charge of a process is 929.5 C?​

Answers

Answer:

5746.25×e.

Explanation:

The electron is known as the subatomic particle which revolves around the nucleus by possessing the negative charge.  

when the atoms are involved in the reaction or process these electrons are transferred are gained by the atoms.  

Coulomb is the term used to express the charge of the electrons.  

In normal 1 coulomb of charge possess  6.25×  electrons.  

So here the total charge of the process is 919.4c in order to find number of electrons transferred is found by the product of  919.4 × 6.25× = 5746.25 ×  .

Thus the number of electrons transferred is obtained. the transferred electrons are 5746.25 ×.

What mass of Iron (III) acetate will be produced when 1.50 grams of iron reacts in acetic acid?

Fe + H(C2H3O2) ® Fe(C2H3O2)3 + H2

Answers

Answer:

Fe + H(C2H3O2) ........

According to the ideal gas law, what happens to the volume of a gas when the pressure doubles (all else held constant)?

Answers

the volume decreases in the same ratio as the ratio of pressure increases so if you double the pressure you will halve the volume

Both magnetic fields and electric fields can exert a force true or false?

Answers

Answer:

false

Explanation:

An electric field may do work on a charged particle, while a magnetic field does no work.

The electrical current in a circuit is measured in which of the following units

Answers

Answer:

joules

Explanation:

i think

Explanation:

The SI unit of electric current is the ampere, or amp, which is the flow of electric charge across a surface at the rate of one coulomb per second. The ampere (symbol: A) is an SI base unit Electric current is measured using a device called an ammeter.

energy is the sum of kinetic energy + potential energy.
(10 Points)
*
Chemical
Electrical
Mechanical
Radiant

Answers

Answer:

electrical

Explanation:

they are working together

Which of the following accurately represents the relationship between ceramic and
metal?

Answers

Relationship between metal and ceramic is accurately represented by the statement that metal is a better conductor than ceramics.

What are conductors?

Conduction is defined as a process as a means of which heat is transferred from the hotter end of the body to it's cooler end.Heat flows spontaneously from a body which is hot to a body which is cold. Substances which enable conduction are called conductors.Conductors allow passage heat and electricity through the material.

In the process of conduction,heat flow is within the body and through itself.In solids the conduction of heat is due to the vibrations and collisions of molecules while in liquids and gases it is due to the random motion of the molecules .

When conduction takes place, heat is usually transferred from one molecule to another as they are in direct contact with each other.There are 2 types of conduction:1) steady state conduction 2) transient conduction.According to the type of energy conduction is of three types:

1) heat conduction

2) electrical conduction

3)sound conduction

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A solvent was composed of two separate components: water and cyclohexane. If ethyl acetate was added to the solvent, into which layer would it most likely dissolve? Explain?

Answers

Answer:

water

Explanation:

In chemistry, an important principle that guides the solubility of a solute in a solvent is the principle of like dissolves like.

A polar solvent will dissolve a polar solute and a non-polar solvent will dissolve a non polar solute.

Ethyl acetate is a polar solute. It will have to dissolve in water which is a polar solvent. Hence, ethyl acetate will dissolve in the water layer.

1. The equation for the reaction between zinc and aqueous copper(II) sulfate is shown.

Zn + Cuso, → ZnSO, + Cu

Which statement is correct?

a. The oxidation state of the oxidising agent has changed from 0 to +2.

b. The oxidation state of the reducing agent has changed from 0 to +2.

c. The oxidation state of the reducing agent has changed from +2 to 0

d. This is not a redox reaction. The solution changes from colourless to blue.

Answers

Answer:

The oxidation state of the reducing agent has changed from 0 to +2.

Explanation:

reducing agent is anything that loses electron or gains oxygen

in this case, zinc

Discuss why it is not necessary to know exactly how much salt was added to the water in making the salt solution to just float the egg?

Answers

Answer and Explanation:

It is not necessary to know the amount of salt in the aqua, because we know that the presence of salt in the water will increase its density.

In this case, salt increases the mass of the water, without increasing the volume of water. as the egg will not change in volume or water, we know that the density of the egg will be constant, while the change in the mass of the water with the addition of salt, will change the density of the water which will increase progressively until it becomes denser than the egg and, consequently, it will not let the egg sink.

who is the father of phycis

Answers

Galileo Galilei

hope it helps you

Answer:

Galileo Galilei is the father of Physics

I hope it helps ❤❤

What a main difference between Earth and Jupiter?

A. Earth is an inner planet with a solid rock surface composition, while Jupiter is an outer planet with a gas surface composition.

B. Earth is an inner planet with a relatively long distance from the sun, while Jupiter is an outer planet with a relatively short distance from the sun.

C. Earth is an outer planet with a gas surface composition, while Jupiter is an inner planet with a solid rock surface composition.

D. Earth is an outer planet with a relatively short distance from the sun, while Jupiter is an inner planet with a long distance from the sun.

Answers

Answer: A. Earth is an inner planet with a solid rock surface composition, while Jupiter is an outer planet with a gas surface composition.

Answer: What a main difference between Earth and Jupiter?

Earth is an inner planet with a solid rock surface composition, while Jupiter is an outer planet with a gas surface composition.

Earth is an inner planet with a relatively long distance from the sun, while Jupiter is an outer planet with a relatively short distance from the sun.

Earth is an outer planet with a gas surface composition, while Jupiter is an inner planet with a solid rock surface composition.

Earth is an outer planet with a relatively short distance from the sun, while Jupiter is an inner planet with a long distance from the sun.

Explanation:

Help! I’ll give brainliest if u get it right!

Answers

Answer:

That is the Atomic mass

Explanation:

The element symbol is S, Element name is sulfur, and

Atomic number is 16

Convert 10.0 g of iron (II) sulfate to moles.

please explain how to do this! I don't understand ​

Answers

Explanation:

number of moles =mass /relative molecular mass

n=10g/mr(FeSO4)

N=10/(56+32+16×4)

N=10/152

N=0,066 moles

Following a chemical reaction that produced 5.06 grams of magnesium chloride, the lab report was prepared to
document the results. The expected result was estimated to be 8.85 grams. What are the percent yield and percent
error that are to be included in the lab report?
Percent Yield
Percent Error

Answers

Answer:

1. Percentage yield = 57.2%

2. Percentage error = 74.9%

Explanation:

From the question given above, the following data were obtained:

Actual yield = 5.06 g

Experimental yield = 8.85 g

Percentage yield =?

Percentage error =?

1. Determination of the percentage yield.

Actual yield = 5.06 g

Experimental yield = 8.85 g

Percentage yield =?

Percentage yield = Actual yield /Experimental yield × 100

Percentage yield = 5.06 / 8.85 × 100

Percentage yield = 57.2%

2. Determination of the Percentage error.

Actual yield = 5.06 g

Experimental yield = 8.85 g

Percentage error =?

Percentage error = |Experimental – Actual| / Actual yield × 100

Percentage error = |8.85 – 5.06| / 5.06 × 100

Percentage error = 3.79 / 5.06 × 100

Percentage error = 74.9%

Based on the following equation, how many moles of hydrochloric acid (HCl)are needed to react with 0.44 moles of potassium
permanganate (KMnO4)?
2KMnO4 + 8HCI - 3Cl2 + 2MnO2 + 4H2O + 2KCI

Answers

Answer:

1.76 moles of HCl.

Explanation:

We'll begin by writing the balanced equation for the reaction. This is illustrated below:

2KMnO₄ + 8HCl —> 3Cl₂ + 2MnO₂ + 4H₂O + 2KCl

From the balanced equation above,

2 moles of KMnO₄ reacted with 8 moles of HCl.

Finally, we shall determine the number of mole of HCl needed to react with 0.44 moles of KMnO₄. This can be obtained as follow:

From the balanced equation above,

2 moles of KMnO₄ reacted with 8 moles of HCl.

Therefore, 0.44 moles of KMnO₄ will react with = (0.44 × 8)/2 = 1.76 moles of HCl.

Thus, 1.76 moles of HCl is needed for the reaction.

Jen is conducting an experiment involving a device known as a pith-ball electroscope, shown below.
silk thread
-plastic ball
insulated
stand
The plastic ball of the electroscope hangs in midair and is uncharged. Charges cannot move along the silk thread that holds the
ball in the air
Jen completes the following steps in her experiment
1. She collects three identical, uncharged rubber tubes
2. She holds a magnet to the first tube for 5 minutes.
3. She submerges the second tube in a tank of water for 5 minutes
4. She rubs the third tube with animal fur for 5 minutes.
Jen's teacher tells her that she can use the pith-ball electroscope to see if any of the rubber tubes are surrounded by an electric
field. What should Jen do to test for an electric field surrounding each tube?
O A. bring the magnet close to the plastic ball and note any motion of the ball toward the magnet
OB.
connect the three tubes to each other and see if they attract the plastic ball
C. hold the magnet in direct contact with the tube that was submerged in water
OD. bring each tube close to the plastic ball and note any movement of the ball
12 of 30 Answered
Session Timer: 35:32
Session Score: 33 (4/12)

Answers

Answer:

A) bring the magnet close to the plastic ball and note any motion of the ball toward the magnet

Explanation:

what do you mean by PCM​

Answers

Answer:

Pulse-code modulation

Explanation:

Convection is said to occur in _______ , which includes liquids and gases.

Answers

Convection is a form of heat transfer that occurs in fluids, which would include liquids and gases.

most ponds contain both slat water and fresh water. true or false

Answers

Answer:

False

Explanation:

Freshwater habitats include ponds, lakes, rivers, and streams, while marine habitats include the ocean and salty seas.

false.

it is rare for ponds to have salt water

help po

what is ecosystem?
what is a rainforest?
what are the major parts of rainforest?
what are coral reefs?
what are the different types of coral reefs? ​

Answers

Answer:

ecosystem:a biological community of interacting organisms and their physical environment.

rainforest: a luxuriant, dense forest rich in biodiversity, found typically in tropical areas with consistently heavy rainfall.

major part of rain forest:Most rainforests are structured in four layers: emergent, canopy, understory, and forest floor. Each layer has unique characteristics based on differing levels of water, sunlight, and air circulation.

coral reefs :a ridge of rock in the sea formed by the growth and deposit of coral.

type of coral reefs:fringing reefs, barrier reefs, atolls, and patch reefs

Explanation:

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Explanation:

1)An ecosystem is a community of living organisms in conjunction with the nonliving components of their environment, interacting as a system. These biotic and abiotic components are linked together through nutrient cycles and energy flows.

2)Rainforests are forests characterized by high and continuous rainfall, with annual rainfall in the case of tropical rainforests between 2.5 and 4.5 metres and definitions varying by region for temperate rainforests

3) Most rainforests are structured in four layers: emergent, canopy, understory, and forest floor. Each layer has unique characteristics based on differing levels of water, sunlight, and air circulation.

4) A coral reef is an underwater ecosystem characterized by reef-building corals. Reefs are formed of colonies of coral polyps held together by calcium carbonate. ... They are most commonly found at shallow depths in tropical waters, but deep water and cold water coral reefs exist on smaller scales in other areas.

5)The three main types of coral reefs are fringing, barrier, and atoll

Hope it help ful
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In October 1922, she broke a world record for female pilots by flying her plane to 14,000 feet in altitude. In 1923, she received her International Pilots Licensejust the sixteenth woman in the world to do so. Unfortunately, Amelia had to sell her plane to give her mother money, and for a while, her love of aviation became more of a hobby.But this all changed in 1928 when Amelia was contacted by publisher George Putnam, who she later married. He invited her to be a passenger on a cross-Atlantic flight. Amelia would have preferred to be the pilot, not the passenger, but at the time, flying a plane over an ocean was considered too dangerous for women.The flight was successful, and Amelia became world-famous as the first woman to fly across the Atlantic. However, Amelia was not satisfied. She did not get to operate the controls during the flight and thought of herself as being no more important to the flights success than a sack of potatoes. She was determined to fly on her own.Amelia Reaches StardomIn 1929, Amelia became involved with an organization called the Ninety-Nines, a group that promoted female pilots in the aviation industry. She was the groups first president. In May of 1932, Amelia crossed the Atlantic again, but this time she was at the controls. She made the trip from Canada to Ireland in 15 hours. She received many medals and awards for her achievement.Amelia hoped her success would open doors for women in aviation and in other fields, so she continued flying and setting records. She became the first person to fly across both the Atlantic and Pacific oceans when she flew from Hawaii to California.An Extraordinary MissionEven after proving she was a world-class aviator, Amelia still wanted more. In 1937, she set off on an amazing trip: flying around the world. Amelia started in California but faced trouble along the way. She became ill, her plane needed repairs, and bad weather forced a change in the flight route. She made it to one of her planned stops near Australia, but she never made it to her next destination. People all over the world were following the news of Amelias tripand now she was missing! President Franklin D. Roosevelt conducted a $4 million rescue mission, but Amelia was never found.Amelia hoped that her actions would inspire other women to follow their dreams. One of her lifelong missions was for the world to recognize that women could have the same careers as men. Even though she died at a young age, just 40 years old, Amelia was an inspiration to many.Amelia Earhart is sitting in her plane with her aviation hat and goggles on.How does the narrators point of view influence how Amelia Earharts life is described? A. The narrator sees her as normal and makes her look common and boring. B. The narrator looks at her as a lucky woman and makes her look happy and popular. C. 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