You have successfully engineered E. coli to make human insulin by adding a transgene that has an E. coli promoter and the coding sequence of human insulin. You try to generate a new strain that would make a lot more insulin by making one with 10 copies of the transgene. Although your genetic engineering is successful, your new strain doesn't make much more insulin. What do you think could have gone wrong

Answers

Answer 1

Answer:are you asking a question?

Explanation:


Related Questions

How contipedes differ from millipedes​

Answers

Answer:

Millipedes have two sets of legs per segment positioned directly under their body, while centipedes have one set of legs per segment positioned on the side of their body.

answer:

Here are a few tips to spot the differences: Millipedes have two sets of legs per segment positioned directly under their body. Centipedes have one set of legs per segment positioned on the side of their body. ... Millipedes feast on decomposing plants.

Explanation:

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PLEASE HELP FAST
4. Which choice is a risk factor that may increase chances of abusing alcohol?
1.no access to alcohol
2.no peer pressure
3.alcoholic parent
4.high self-esteem

Answers

Answer:

3.alcoholic parent..ÔwÔ

Which event occurred during the Mesozoic era?


Cyanobacteria filled the water and air with oxygen.


Early humans began to make tools, harvest plants, and make cave paintings.


Pangaea began to break apart into many continents separated by shallow seas.


Pangaea, the super continent, was formed.

Answers

Answer:

Pangea began to break apart.

Explanation:

Humans wouldn't exist for millions more years. Pangea existed before the Mezoic, and the Cyanobacteria doesn't make sense.  (Not an expert though.

what was the speed of a car that traveled 240 m in 12 s

Answers

Answer:20

Explanation:   Because when you divied 240 by 12 you get 20.

240/12=20

Answer:

20m/s

Explanation:

Speed is equal to distance/time. Since we know that the distance is 240m and the time taken to travel 240m was 12 seconds, we can divide them 240/12 and once we do this we can get our final answer of 20m/s.

help me woth these two ill give free BRAINLESS​

Answers

answer : the elbow is an example of the second last one (it won't let me write the word)

Answer:

Answer D

Explanation:

This is a synovial joint, or just D

The possibility of a baby having a certain genetic trait is in the ratio of 3.1. Give the probability of this occurring as a fraction and as a
percentage. Explain.
Tyn your answer below

Answers

Fraction-1/4
Percentage-25%
Because In a punnet square there are 4 squares and in a 3:1 ratio it would mean one of the squares is affected by the genetic trait so it would be 1/4. And 1/4 is 25%.

When we say that a bulb is fused, what is wrong with the bulb? How can we use it again?​

Answers

Hey mate !

here is ur answer(refer the image)

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Simple organisms usually make exact copies of themselves when they reproduce. What do we call this type of reproduction?​

Answers

Answer:

asexual reproduction.

What is a phenotype that is influenced by the environment called?


Answers

I don’t have time hf had hjgfhgdhtekhgm. Fjggjlfkhf,jikuf,nvm how.

Which of the following correctly explains the differences between artificial and natural selection?

Answers

Not sure what the options are, but artificial selection would be when farmers/breeders choose only to plant/breed the organism with desired characteristics in order to cause the evolution of the trait while natural selection is when organisms that are better adapted to their environment usually survive and produce offspring, causing the evolution of their traits which help them to survive.

Hope that helps!

Inversion of DNA sequences within chromosomes is a common process in evolution. The following gene arrangements in a particular chromosome are found in four different species: First sequence: STUVWX Second sequence: UVXTSW Third sequence: UVWSTX Fourth sequence: SWVUTX Assuming that the arrangement in the first sequence is the ancestor/reference arrangement, in what evolutionary order did the four species arise, such that the fewest number of inversions occur between each species (place the first sequence as the earliest species in your ordering)2341

Answers

Answer:

The correct answer is - 1, 4, 3, 2.

Explanation:

It is given that the first sequence represents the earliest or ancestor species therefore, the first sequence would be - STUVWX

After the inversion, the highlighted sequence of the first sequence STUVWX gives rise to the second sequence as:

STUVWX

SWVUTX

Thus the second sequence after Ist inversion would be SWVUTX

2nd inversion, Third sequence:

SWVUTX

UVWSTX

Thus the third sequence after 2nd inversion is UVWSTX

3rd inversion, fourth sequence:

UVWSTX

UVXTSW

Thus, the fourth sequence after 3rd inversion is UVXTSW

Which question cannot be tested with Scientific investigation?

Answers

Answer:

air

Explanation:

the structure labeled A in the figure below is called the

Answers

Answer:

Centromere

Explanation:

Connects sister chromatids

The structure labelled A in the figure is a centromere.

What is a centromere?

The centromere is a region of the chromosome that is constricted. The centromere plays an important role in the division of cells during both the processes of mitosis and meiosis.

The centromere is simply the region of the chromosome where the spindle fibres attach. Therefore, when the spindle fibres attach to the centromere, they pull the sister chromatics in the opposite directions of the cell during cell division.

Thus, at the end of the cell division, each of the daughter cells gets one-half of the chromosome containing identical DNA. The centromere is thought to be the middle region of the chromosome, even though it is often not in the middle. It is simply the region where the chromosomes are most constricted

The centromere separates the chromosome into the P arm and the Q arm. These are simply the names given to the short arm and the long arm of the human chromosome respectively.

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What does it mean if a galaxy gives off light that has been shifted toward the red
end of the electromagnetic spectrum?

Answers

Answer: Redshift and blueshift describe how light shifts toward shorter or longer wavelengths as objects in space (such as stars or galaxies) move closer or farther away from us. ... When an object moves away from us, the light is shifted to the red end of the spectrum, as its wavelengths get longer.

Explanation:

Use the cladogram to answer the question. Which trait(s) does the salamander have?
Lancelet
(outgroup) Lamprey Tuna Salamander Turtle
Leopard
Hair
Amniotic e99
Four walking legs
Jaws
Vertebral column
amniotic edd and hair
vertebral column, jaws, and four walking legs
four walking legs only
vertebral column and jaws only

Answers

Answer:

everything plesiomorphic (ie ancestral) so 4 walking legs, jaws and vertebral column

Explanation:

prostate cells usually require testosterone and other androgens to survive. but some prostate cancer cells thrive despite treatments that eliminate androgens. one hypothesis is that estrogen, often considered a female, may be activating genes normally controlled by an androgen in these cancer cells. desribe one or more experiments to test this hypothesis

Answers

One can check for the translation and transcription of genes of interest in the absence of androgens, one can check whether the estrogen is involved in the expression of oncogenic proteins of interest by depleting the estrogen, and one can also examine the existence of estrogen receptor complex in the prostrate cancer cells nuclei.  

• One can check for the translation and transcription of genes of interest in the non-existence of androgens and if these genes are expressed then this suggests that apart from androgens other factors also affect their expression.  

• One can deplete the estrogen from cancer cells and if the involvement of estrogen is there in the expression of oncogenic proteins of interest then its reduction will result in diminished multiplication rates in cancer cells.  

• One can also test the existence of estrogen-receptor complex in the prostate cancer cells nuclei to confirm that estrogen is taking part in the regulation of gene expression.  

Thus, various experiments can be conducted to check that whether the expression of genes controlled by the androgens under normal conditions are affected by the presence of estrogens in prostate cancer cells.  

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why is vachellia nilotica classified as family level

Answers

Answer:

Vachellia nilotica is a flowering plant tree in the family Fabaceae.

Explanation:

describe the feature of cell mbrane​

Answers

Answer:

The cell membrane or plasma membrane is a biological and thin semi-permeable membrane that surrounds the cytoplasm of a cell

Explanation:

which of the following is not a condition that must be made for hardy-weinberg equilibrium 1.no mutation 2.no migration 3.domunant alleles nore frequent than recessive allels 4.large population 5. no natural selection guy please answer this one​

Answers

Answer:

3.dominant alleles more frequent than recessive alleles

Explanation:

To achieve hard-Weinberg equilibrium, certain conditions must be made.

These conditions are:

1. There is noo mutation,

2. Existence of random mating,

3. There should be no natural selection

4. There is the infinite population size

5. No migration

Hence, in this case, the correct answer is option 3, which is not a condition to be made for Hard-Weinberg equilibrium


1. Explain how the atomic number and mass number of an atom can be used to determine the number of
electrons and of neutrons respectively.

Answers

Because the total of neutron and proton/electron is the atomic mass. So if you wanna find proton/electron and you know the neutron, just minus the atomic number with the neutrom and you will get the proton

Here if you need the formula

Atomic number = Total neutron + Total proton or electron

Need some quick help with this:

Answers

Answer:

In photosynthesis, solar energy is harvested as chemical energy in a process that converts water and carbon dioxide to glucose. Oxygen is released as a byproduct. In cellular respiration, oxygen is used to break down glucose, releasing chemical energy and heat in the process.

Explanation:

Answer:

option C

Explanation:

radiant energy from the sun get converted into chemical energy during the photosynthesis process by the chlorophyll .

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During glycolysis, the molecule glucose is broken down into pyruvate. What type of reaction is this?

Answers

Explanation:

Glycolysis is a set of anaerobic reactions, ones that does not need oxygen to be performed. It takes glucose and breaks it down into pyruvate. This has the result of creating two ATP, which can release energy.

Overall, this process creates four ATP, but it uses two ATP early in the reaction. This means that there is a net gain of two ATP.

The overall equation for this pathway is:

1 glucose + 2 ATP ⟶ 2 pyruvate + 2 NADH + 4 ATP + 2 H2O

Glycolysis is a set of anaerobic reactions, ones that does not need oxygen to be performed. It takes glucose and breaks it down into pyruvate. This has the result of creating two ATP, which can release energy.

Overall, this process creates four ATP, but it uses two ATP early in the reaction. This means that there is a net gain of two ATP.

What does glycolysis process?

Glycolysis is the method of breaking down glucose. Glycolysis can take place with or without oxygen. Glycolysis produces two molecules of pyruvate, two molecules of ATP, two molecules of NADH, and two molecules of water.

Glycolysis occurs in the cytoplasm.

Thus, Glycolysis is the method of breaking down glucose. Glycolysis occurs in the cytoplasm.

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propose two ways the body temperature of a sick person can be brought to normal​

Answers

Answer:

ice pack

Explanation:

Answer:

Swimming in cool water, taking a lukewarm bath, or applying cold water to the body can reduce body temperature.

Explanation:

hope this helps you!

which of the following is the most complex
according to their cellular level of organization? A.
Hair B. Euglena C. Hydra D.Heart. bplease give explanations.​

Answers

d.corazon.ya que sin el no habria vida


A helium atom that has only two electrons will have its electrons in which orbital(s)?

Answers

1s orbital

Explanation:

The electron has to go into the first energy level, in the 1s orbital. Helium, with two protons, will have two electrons. These are placed one at a time into the 1s orbital. This makes helium a very contented atom.

brief explain the roles of the following in photosynthesis 1 NADP 2) Ribubose diphostate 3) photosytem 1 ad 2​

Answers

Answer:

1) NADP is the reducing agent produced by the light reactions of photosynthesis and is consumed in the Calvin cycle of photosynthesis and used in many other anabolic reactions in both plants and animals.

3) Photosystems are the functional units for photosynthesis, defined by a particular pigment organization and association patterns, whose work is the absorption and transfer of light energy, which implies transfer of electrons. Physically, photosystems are found in the thylakoid membranes. There are two kinds of photosystems: photosystem I (PSI) and photosystem II 

● PhotosystemSII acts first during the light transformation process in photosynthesis.

● Photosystem1 is an integral membrane protein complex that uses light energy to catalyze the transfer of electrons across the thylakoid membrane from 

plastocyanin to ferredoxin. Ultimately, the electrons that are transferred by carrier NADPH.

what is peace and conflict??​

Answers

Peace and conflict studies is a social science field that identifies and analyzes violent and nonviolent behaviours as well as the structural mechanisms attending conflicts (including social conflicts), with a view towards understanding those processes which lead to a more desirable human condition.

A force of 5 N is required to increase the speed of a box from a rate of 1.0 m/s to 3.0 m/s within 5 s along a level surface. What change would most likely require additional force to produce the same results?
(SC.6.P.13.3)
reduce the mass of the box
increase the mass of the box
make the surfaces of the box smooth
make the surface of the floor smooth


HELPPP

Answers

Answer:increase the mass of the box

Explanation:

Big brain

What 3 types of science formed the geological time scale?

a) archaeology, paleontology, astrology
b) Scientology, astronomy, and geology
c) geology, archaeology, and paleontology
d) paleontology, Scientology, and biology

Answers

Answer: c) geology, archaeology, and paleontology

Explanation:

The geological time scale can be defined as a scale which is used to determine the chronological dating of the organisms in the geological strata. It helps in determining the occurrence and existence of particular kind of species on planet earth. It can also be used to trace the development and passage of a particular kind of trait from one generation to the another. It can also be used to trace back various geological events that occurred on planet earth like volcanic eruption, plate tectonics, and others. The geologists used it for tracing the geological activities that occurred in the history of earth. Archaeologist study about rocks and fossils to trace back the geological time scale. Paleontologist study the existence of a particular species via studying the fossil evidences and reconstructing the primitive conditions by developing a geological time scale. They trace the evolutionary history of organisms.

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Which best describes how limiting factors affect all the species in this ecosystem?

Answers

Answer:

Its a COYOTE

Explanation:

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