You do a serial dilution in the same manner as the one in the video. A plate with a sample volume of 10-6 mL produced 43 colonies. What was the original cell density (CFU)?

Answers

Answer 1

Answer: 43 x 10^6  CFU.

Explanation:

Plate count is a method used to determine the number of viable microorganisms in a sample. When the bacterial concentration is high, serial dilutions are carried out in a 1:10 sequence. Then, the dilutions are successive dilutions keeping the dilution factor constant at each step. Small aliquots of these dilutions are seeded in medium contained in a Petri dish where the bacteria grow forming colonies. Then the plates are incubated and colonies develop both within the agar and on the surface.

If the concentration of the seeded aliquot is too high, the bacteria will grow too much and it will not be possible to distinguish the colonies. However, if the concentration is very low, the number of colony forming units (CFU) may be very low and thus can be counted.

Colony forming units are calculated:

CFU = Number of colonies / (volume seeded on the plate * dilution).

The dilution is 10^-6, the number of colonies counted is 43, and usually the volume plated is 0.1 mL, so we replace that in the equation:

CFU= 43 / (0.1 mL * 10^-6)

CFU= 43000000 = 43 x 10^6


Related Questions

The evidence Alfred Wegener used for his continental drift hypothesis did not include glacial movement. types of rocks and mountain ranges. oceanic vocanoes. fossilized remains of ancient plants.

Answers

Answer:

oceanic vocanoes

Explanation:

The continental drift is an idea that the continents can move. Alfred wegner in his evidence of this phenomenon used the following hypothesis:

Rocks of same age and type formed side by side and these rocks drifted apart to separate continents. Also as continents drifted apart mountain ranges separated.

Alfred used movements from ancient glaciers as evidence,

Fossils of dead plants and ancient animals also served as evidence. The fossils of these plants and animals were found on land areas that are now far apart. Wegner concluded that the continental drift was what separated the remains of these fossils.

All of the above was used as evidence. What was not used as hypothesis by Wegner was oceanic volcanoes

Wegener used fossil evidence to support his continental drift hypothesis. The fossils of these organisms are found on lands that are now far apart. Grooves.

What is the mRNA made from the following DNA.
CGATCG?
(please help!)
A. CGTUCG
B. CGATCG
C. GCTAGC
D. GCUAGC

Answers

Answer:

GCUAGC (D)

Explanation:

DNA always consists of A/T and C/G

RNA (mRNA/tRNA always consists of A/U and C/G but never T!

If you have a T in your DNA, your mRNA will have an A.If you have an A in your DNA, your mRNA will have a U.

DNA never has a U, but RNA never has a T.

Hope this makes sense!

respiration is a ____________ reaction on which sugar is oxidized to produce energy ​

Answers

Answer:

chemical

Explanation:

Respiration is a redox reaction on which sugar is oxidized to produce energy .

What is respiration?

Cellular respiration is a sequence of chemical events that break down glucose to produce ATP, which can then be utilized as energy to power a variety of reactions throughout the body. Glycolysis, the citric acid cycle, and oxidative phosphorylation are the three basic processes that take place during cellular respiration.

Animals and humans use breathing to get the oxygen they need for cellular respiration. In order to create ATP, the nutrients you absorb through food must be broken down by the oxygen you breathe, while carbon dioxide and  water are generated as waste products.

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Are physical characteristics of an organism not genetically passed down?

Answers

That is false. Physical characteristics of an organism are genetically passed down.

—Evidence—

A characteristic that an organism can pass on to its offspring through its genes.

The set of information that controls a trait; a segment of DNA on a chromosone that codes for a specific trait.

Heredity is the biological process responsible for passing on physical traits from one generation to another.

Si fuimos creados para la felicidad ¿Por qué la mayoría de las personas viven sus vidas de manera infeliz? Escribe por las menos 3 razones

Answers

Answer: esto se deve a la monotonia por ejemplo el trabajo hacer eso todos los dias es estresante

tambien puede ser devido a problemas personales por ejemplo un divorcio la muerte de algun familiar.

o pueden ser las condiciones de vida que se poseen

Explanation:


Which of the following has the highest reproductive potential?
O a. bacteria
O b. cattle
O c. humans
O d. elephants

Answers

Answer:

A. Bacteria

Explanation:

I asked my teacher hahahahahahahaha

Bacteria as the highest reproductive potential. Hence option a is correct.

What is reproductive potential?

Reproductive potential is defined as the proportional ability of a species to reproduce itself under ideal circumstances. The maximum number of children that a particular organism can produce is known as its reproductive potential. Compared to other species, some have substantially higher reproductive capacity.

When people reproduce more frequently, sooner in life, and at higher rates, their reproductive potential rises. The largest influence on reproductive capacity is early pregnancy. Every living thing must be able to reproduce. All living things produce additional organisms that resemble them.

Thus, bacteria as the highest reproductive potential. Hence option a is correct.

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8.What attaches muscles to bones?

veins
tendons
ligaments
joints

Answers

Tenons attach the muscles to the bones


5. Using the picture below, fill in the correct word that corresponds with each letter
A.
B
В.
C
D
6CO2 + 6H20,+ Energy,
C6H12O6 + 602
+
F
C
D
E
F
G
GO
H
This is the formula for:
H Н.

Answers

Answer:

ano yan depota

Explanation:

pota pota

How are single double and triple bonds similar

Answers

Answer:

Double and triple covalent bonds are stronger than single covalent bonds and they are characterized by the sharing of four or six electrons between atoms, respectively.

Explanation:

BEST ANSWER GETS BRAINLYEST
When talking about the products of photosynthesis, why is it a HUGE issue when a vast amount of the rainforests is dying along with vast amounts of forests from fires/deforestation? Remember, we know that this hurts the ecosystems of animals living there, but what else does it effect?

Question 5 options:

the creation of carbon dioxide


the creation of carbon monoxide


the creation of too much smoke


the creation of oxygen

Answers

Answer: the creation of oxygen

Explanation:

took the K12 Quiz (Note: not to sure its right if it aint so so sorry!)

All the information necessary for an organism to form and grow is recorded in the organism’s DNA. How does DNA use its stored information to express a trait?

A. Every time the DNA replicates, traits are expressed.
B. The nucleotides in the DNA bind with amino acids in order to direct the cell's
functions.
C. Codons in the DNA stimulate the DNA to replicate.
D. The genes in the DNA instruct the cell to synthesize proteins.

Answers

mm I think it is number 3.

Answer:

it's B The nucleotides in the DNA bind with amino acids in order to direct the cell's

functions.

Explanation:

Glomerular filtrate comprises of water, glucose, amino acids and creatine. Which among the above are reabsorbed actively and rapidly by blood?

Answers

Answer:

Glucose, amino acids are rapidly reabsorbed actively by blood.

Explanation:

What can you conclude about the effect of exercise on breath rate? Why is this so?

Answers

Explanation:

With exercise, cells need more oxygen, so the breath rate increases to provide the oxygen needed

PLEASE I NEED HELP ILL GIVE BRAINIEST !!!!

Answers

Answer:

the correct sequence of arrangement of level of ecological organization from least to most inclusive is organism,population, community

Answer:

Its below in the explanation

Explanation:

Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species.

Since species is the widest category.

What are created during the process of translation?

Answers

Answer:

a gene expression

Explanation:

and

why do all the planets in the solar system orbit the sun in the same direction ?

Answers

Answer:

The result of the initial rotation of the cloud of gas and dust that condensed to form the Sun and planets. As gravity condensed the clouds, of angular momentum increased the rotational speed and flattened the cloud out into a disk.

A liquid is matter that
A. has a definite volume and takes the shape of its
container.
B. has a definite shape and won't take the shape of its
container.
C. has no shape, and its volume changes randomly.
D. has a volume that changes with its container.

Answers

Answer:

272939

Explanation:

373773737384848

Answer is D =). Ur welcome sowwy if I’m wring

When a plant or animal is no longer found anywhere on Earth, it is described as
being
locally extinct
geographically extinct
biologically threatened
biologically extinct

Answers

Answer:

B. Geographically extinct

Explanation:

Lets take a moment to look at the words provided. Geographical means physical features of an area, or relating to geography. It can also relate to when we are talking about information on the Earth's surface. Extinct means that the organism is no longer alive, and that there are none left of its species. So, putting the two words together, geographically extinct means that the organism is no longer found on Earth.

Hope this helps!

Panna

When compared to a cheek cell, a muscle cell would contain more

Answers

Answer:

Chromosomes

Explanation:

Waves turn rocks into ___________________; it’s erosion.

Answers

Answer:

sand?

Explanation:

[tex]\huge{\textbf{\textsf{{\color{pink}{An}}{\red{sw}}{\orange{er}} {\color{yellow}{:}}}}}[/tex]

when waves turn rocks in to smaller rocks or sand... it's erosion

ILL GIVE U BRAINLYIST

Answers

Answer:

D.

Explanation:

That is where it demonstrates how the word is said. it's easy to identify this because the pronunciation always comes right after the word itself in the definition.

Answer:hbhh

FffcffExplanation:

effect of time on the digestion of starch

Answers

Answer:

60 minutes im pretty sure

DNA is an example of a nuclei case acid true or false

Answers

Answer:

The term nucleic acid is the overall name for DNA and RNA.

Explanation:

They are composed of nucleotides, which are the monomers made of three components: a 5-carbon sugar, a phosphate group and a nitrogenous base. ... The two main classes of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).

Your body uses feedback mechanisms to help maintain a stable internal environment called homeostasis. Changes can come from outside or inside the body and the body must respond to these adverse changes to return to a steady state. One such example is what happens when you go for a run on a hot summer day and your body temperature increases. According to the diagram, one of the mechanisms used to cool down is vasodilation. Similar mechanisms are also used when your body temperature drops due to a cold environment. Which of these would NOT help return a body to homeostasis by increasing body temperature?
A) shivering
B) vasoconstriction
C) muscles contract
D) increasing blood pressure

Answers

Answer:

Option D increasing blood pressure.

Explanation:

Increasing blood pressure

What is feedback mechanism in homeostasis?

Homeostasis is generally maintained by a negative feedback loop that includes a stimulus , sensor , control center , and effector . Negative feedback serves to reduce an excessive response and to keep a variable within the normal range.

What are the two feedback mechanisms which work to maintain homeostasis?

Homeostasis typically involves negative feedback loops that counteract changes of various properties from their target values, known as set points. In contrast to negative feedback loops, positive feedback loops amplify their initiating stimuli, in other words, they move the system away from its starting state.

To learn more about homeostasis here

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Some research studies claim that a person with antisocial personality disorder will not have a(n) __________ in stress hormone levels when under __________.

decrease . . . medication
increase . . . stress
decrease . . . stress
increase . . . medication

Answers

Answer:

B. Increase...stress

Explanation:

Correct on Edg 2021

Some research studies claim that a person with an antisocial personality disorder will not have a(n) increase in stress hormone levels when under stress. Option B.

What is an antisocial personality disorder(Disease)?

Generally, An antisocial personality disorder is simply a personality disorder marked by persistent antisocial, irresponsible actions

In conclusion, Patients are always violent, with disdain for any hurt or pain given to others, and an inability to sustain long-term social and personal connections.

Read more about Disease

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Scientists study telomeres in order to understand more about

Answers

Answer:

They study telomeres to understand the structure of life and death when cells and organs wear out.

Explanation1:

It's the specific DNA of protein structured found a both ends of each chromosome

Explanation2:

Definition: A compound structure at the end of a chromosome.

NEED ANSWER ASAP

Students in a Biology class were formulating an argument about a cell they viewed under the microscope. Half
of the class argued it was a plant cell and the other half argued in favor of an animal cell. Below is the image the
students saw under the microscope. Which argument is supported by the image?

A. The students who said the plant cell are correct because there are chloroplasts

B. The students who said a plant cell are correct because there are mitochondria present.

C. The students who said animal cell are correct because there are chloroplasts present.

D. The students who said an animal cell are correct because there are mitochondria present.

Answers

Answer:

the answer would be A

Explanation:

Animal cells are oval and egg shaped so we can cancel out c and d

A is the correct answer because we can see green dots within the structure. Chloroplasts contain a green pigment called chlorophyll. This green pigment makes the chloroplast and the structure green (as we can see in the picture). Photosynthesis occurs in the chloroplasts, and it is where the plant gets its glucose from

Hope this helps!

HELP ME FAST SMART PEOPLE PLEASE HEHE LOL:)

Answers

Answer:B

Explanation:

Asorbs the heat

PLS HELP ME ASAP 50 POINTS EACH!!

Having dimples is a dominant trait. Having no dimples is recessive.

A father has the genotype Dd and a mother has the genotype Dd.

What is the correct phenotypic possibilties for their offspring?

100% DD, 0% Dd, 0% dd

Answers

E is the answer hope it helps

Answer:

option D

. I hope this helps

What happened at point B? Why?

Answers

Answer:

population has decreased over that period ,MAyBe

Explanation:

I don't know why

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