To assist in completing this question, you may reference the Animated Technique Video - Gel-Filtration Chromatography. Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa. Arrange the molecules in order of their elution from a gel filtration column

Answers

Answer 1

Answer:

IgG molecule, p53 molecule, actin molecule, histone molecule

Explanation:

In a Gel-Filtration chromatography, the larger molecules elude faster from the gel than the small ones.

In a Gel-Filtration chromatography, there is a stationary phase and a mobile phase. The stationary phase has beads with pores. Small molecules enter the beads and descend through these beads' pores. On the other hand, large molecules do not enter the beads' pores, so they go down through the mobile phase faster than the small molecules. Since they do not have the obstacle of the pores, they descend between the beads more fluidly. As a result, the larger molecules elute first from the gel than the small molecules.


Related Questions

What is a benefit engineers have seen as a result of improving the mutation process?

A. Engineers have total control to prevent mutations from occurring.

B. Engineers have an abundance of seeds with different genetic viruses.

C. Engineers have figured out how to use the mutation process on machines.

D. Engineers do not need to grow seeds into adulthood in order to identify the
mutations.

Answers

Answer:

D. Engineers do not need to grow seeds into adulthood in order to identify the mutations.

Explanation:

Imagine that you are walking in the woods for fun and all of a sudden you see amountain lion. Your hair stands up, you begin to sweat and your heart beataccelerates as you prepare to run away. What fueled the energy for the run?

a. The binding of epinephrine to tyrosine kinase receptors and consequent initiation of a response that leads to muscle contraction that allows you to run
b. The activation of a G-protein receptor that leads to the secretion of a protein that makes you run.
c. The binding of epinephrine to a G-protein coupled receptor with the subsequent activation of a nuclear receptor.
d. The initiation of a signaling mechanism that leads to breakdown of glycogen into glucose that can then serve as the fuel for ATP production.
e. All of the above

Answers

Answer:

The correct answer is - D. The initiation of a signaling mechanism that leads to the breakdown of glycogen into glucose that can then serve as the fuel for ATP production.

Explanation:

In challenging or dangerous conditions, the body releases a hormone called Epinephering which is a fight or flight hormone that provides a high amount of energy for the particular action.

Epinephrine hormone binds to beta-adrenergic receptors present on muscles and responsible for the rapid conversion of glycogen to glucose-1-phosphate. The glucose results in condition to rapid synthesis of ATP in muscles and thus helps in muscle actions.

Comment on the folding of protein in vaccum​

Answers

Answer:

Protein folding is a process by which a polypeptide chain folds to become a biologically active protein in its native 3D structure. ... The amino acids in the chain eventually interact with each other to form a well-defined, folded protein. The amino acid sequence of a protein determines its 3D structure.

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It is the four minaret tower. The city of Hyderabad is designed around it, and is often referred
as the 'Arc of Triumph of East.____________​

Answers

Answer:

it is Charminar..... i guess!!!

Explanation:

Which statement describes an interaction between the biosphere and the atmosphere that is related to
photosynthesis?
O During photosynthesis, plant roots take in water from soil.
O During photosynthesis, plants take in carbon dioxide from the
air.
O Through photosynthesis, energy stored in plants is released into the air.
• Through photosynthesis, energy stored in plants is transferred to
humans who eat them.

Answers

Answer:

the second problem of course because, Plants need carbon dioxide and water from air and soil.

Explanation:

O

Answer: The answer is: During photosynthesis, plants take in carbon dioxide from the

air.

Explanation: I have my ways ;>

a cross that results from a yellow and a purple allele together will result in what color corn kernel?

1.) yellow
2.) purple
3.) orange
4.) doesn’t exist

Answers

Answer:

1.) yellow

Explanation:

The high possibility of corn kernel colour is yellow if the yellow colour allele is dominant because the dominant trait or allele always appear in the offspring while on the other hand, the recessive allele does not appear in the shape and structure of the offspring so we can say that dominance is the reason for the appearance of colour in the seed or kernel.

The least genetic variation will probably be found in the offspring of organisms that reproduce using

Answers

Answer:

The least genetic variation will probably be found in the offspring of organisms that reproduce using asexual reproduction.

In the descendants of organisms that use mitosis to build a bigger population, the least genetic variety will likely be observed.

What is mitosis?

Mitosis results in an increased population. Unlike other types of animals that were present at the time, woolly mammoths became extinct thousands of years ago.

Numerous processes can introduce genetic variety, such as mitosis alterations, meiosis crossing over and random orientation, and random fertilization.

All mitotic children share a similar genetic makeup since the duplicate chromosomes' genes are passed down to each succeeding cellular generation.

Each gamete has a unique DNA set because of meiosis's autonomous sorting and recombination. In the resultant zygote, this results in a unique mix of genes.  

Therefore, in organisms that use mitosis to build a bigger population, the least genetic variety will likely be observed.

Learn more about mitosis, here:

https://brainly.com/question/29776367

#SPJ2

Please fast i need an answer!

Answers

Answer:

B

Explanation:

the nerve endings are exposed

Formaldehyde is an organic compound. Each molecule includes two hydrogen (H) atoms, one oxygen (O) atom, and one carbon (C) atom. Below are the dot structures for each of these elements.

At left, a purple circle labeled H surrounded by two concentric circles, the inner with nothing on it, the outer with 1 small green spheres. In center, a purple circle labeled O surrounded by two concentric circles, the inner with 2 small green spheres, the outer with 6 small green spheres. At right, a purple circle labeled C surrounded by two concentric circles, the inner with 2 small green spheres, the outer with 4 small green spheres.
Which diagram shows how the covalent bonds most likely form in a formaldehyde molecule?

C connected above and below to H's by 1 line each. C also connected by two lines to O, which has 2 dots each above and below.

C connected above and below to H's by 1 line each. C also connected by three lines to O, which has 2 dots each above and below.

O connected above and below to H's by 1 line each. O also connected by two lines to C, which has 2 dots above.

O connected above and below to H's by 1 line each. O also connected by two lines to C, which has 2 dots each above and below.

Answers

Answer- H

                |     ..

               C = O

                |     ..

               H

Explanation: Correct on Edg 2020/2021.

Answer:

A.

Explanation:

What are the common features of the favorite careers in your class?

Answers

Answer:

what are some favorite careers in your class?

Explanation:

i don’t have any lol

Scientists realized each person's DNA is different in 1984.

True
False

Answers

True!

By the 1940s scientists realised that DNA contained the code for life, and in 1953 Watson and Crick at Cambridge University discovered how information was stored in DNA and transferred to the next generation. In 1984 Professor Alec Jeffreys discovered the variations in DNA, unique to each individual.

The study of chemical reactions in cells is useful in the analysis of metabolism. What is metabolism?

Answers

Answer:

metabolism refers to all chemical reactions that allow to the cells carry out their functions

Explanation:

In a wide sense, the term metabolism refers to the chemical reactions that occur in the cells of a particular organism in order to sustain life. In a cell, the term metabolism can be delimited as all the chemical reactions inside the cell in order to carry out all cellular functions (e.g., growth, development, reproduction). Metabolic reactions can be divided into 1-catabolic reactions, which typically release energy and involve the breakdown of larger molecules into simpler ones, and 2-anabolic reactions, which typically require an input of energy stored in the chemical bonds of complex molecules.

E

Since the 1960s, the Medicare and Medicaid programs have successfully

reduced discrimination against minorities in renting and housing.

increased the number of college graduates among all groups.

reduced the amount of military spending in the national budget.

O increased the number of people who have health insurance.

Answers

Answer:

The correct answer is - increased the number of people who have health insurance.

Explanation:

Medicare and Medicaid are the federal health insurance programs introduced during the early 60s to cover all the expenses on the health issues of people over 60 or above. It is controlled by the federal agency.

It is increased in great number since the 1960s which helps elderly people of the US to cover all the expenses they spend on their health issues and diseases.

Since the 1960s, Medicare and Medicaid programs have increased the number of people who have health insurance.

How does Medicare work?

Established in 1966, Medicare is funded by Social Security and provides care for people over 65 who have contributed health tax payments during their working years. It also provides coverage for people with disabilities or conditions that prevent them from working.

From this information, we can conclude that since the 1960s, Medicare and Medicaid programs have increased the number of people who have health insurance.

Learn more about health in brainly.com/question/13179079

Discuss how population size and natural events could limit the resource availability in an ecosystem.

Answers

As population size increases, more resources, like food, are needed to satisfy them. This would decrease the availability. Natural events like hurricanes could wipe out the environment and resources that are needed for the organisms, thus limiting them.


How is the organism that receives the DNA with the code for the desired trait
described?

1.genetically modified

2.the receiver organism

3.the strongest organism

Answers

I think it’s 2 I’m sure
It would be 1. genetically modified. Since they are inserted desired genes into the bacteria, they are modifying it.

Left handed DNA
2 points
O a) A-DNA
O b) B-DNA
O c) Z-DNA
O d) C-DNA​

Answers

Z-DNA is one of the many possible double helical structures of DNA. It is a left-handed double helical structure in which the helix winds to the left in a zigzag pattern, instead of to the right, like the more common B-DNA form.

[tex] \: \: \: \: \: \: \: \: hope \: it \: helps \: [/tex]

Answer:

c

Explanation:

z-dna is a left handed DNA

what's DNA is copied and cell prepares to divide phrase​

Answers

Answer:

Mitosis is the process in which a eukaryotic cell nucleus splits in two, division of the parent cell into two daughter cells. The word "mitosis" means "threads," and it refers to the threadlike appearance of chromosomes as the cell prepares to divide.

You arrive at the scene of an accident where people are panicked and yelling. Three people appear to be injured. What action will you take FIRST?

Answers

Answer:

Go over to the panicked people and ask what happened.

Explanation:

I hope this helps you!

possibly call an ambulance or attend to anyone who seems to be going unconscious or bleeding profusely

Another aspect of the competitive exclusion principle is that animals that compete within the same environment may eventually come to occupy their own NICHE. A niche is a way of life, and includes how the animal lives, what it eats, reproduction strategies and more. For example, a hawk and owl both eat the same kinds of food, but the owl hunts at night and the hawk during the day. How did your final ecosystem?

Answers

Answer:

Both hawk and owl share the same habitat or ecosystem.

Explanation:

According to competitive exclusion principle, two species can't coexist in the same environment if they occupy exactly the same niche but in the case of hawk and owl, it is possible because both have different times of eating habits. Hawks hunts at daytime while on the other hand, an owl hunts at night so both share the same habitat due to difference in their niche and they are present in the same ecosystem.

Which of the following is not one of the bases of DNA?

Adenine
Cytosine
Ribosine
Guanine

Answers

I am pretty sure it’s Ribosine

Explain why the resistance of the current path through the extracellular fluid is much smaller than the resistance of the current path through the axoplasm?

Answers

Answer:

The correct answer is - the large cross-sectional area and greater length of the cytoplasmic core get less resistance than the smaller cross-sectional area.

Explanation:

The greater length and the large cross-sectional area of the cytoplasmic path or core get less resistance than the resistance of the current path which is the small cross-sectional area of axoplasm. This leads it to greater resistance than the resistance of the current path through the extracellular fluid.

Other than this there is also an unequal distribution of the ions that leads to the increase in potential difference as higher Na+ ions present in cytoplasm and high amount of K+ ion present in axoplasm.

A student is studying the levels of organization in living things and creates a table to give examples of the different levels.
Which of these should the student write as an example of the organism level of organization?
A. sparrow
B. stomach
C. muscle tissue
D. culture of bacteria

Answers

A. Sparrow just took it on edg!

Answer:A. sparrow

Explanation:

what are the two strands of DNA

Answers

Answer:

The DNA molecule consists of two strands that wind around one another to form a shape known as a double helix. Each strand has a backbone made of alternating sugar (deoxyribose) and phosphate groups. Attached to each sugar is one of four bases--adenine (A), cytosine (C), guanine (G), and thymine (T). But there are two types of DNA in the cell – autosomal DNA and mitochondrial DNA. Autosomal DNA (also called nuclear DNA) is packaged into 22 paired chromosomes. In each pair of autosomes, one was inherited from the mother and one was inherited from the father.

Extra: Because the two strands of a DNA molecule have complementary base pairs, the nucleotide sequence of each strand automatically supplies the information needed to produce its partner. Before replication can occur, the length of the DNA double helix about to be copied must be unwound.

Answer:

The two DNA strands are the DNA molecule consists of two strands that wind around one another to form a shape known as a double helix. Each strand has a backbone made of alternating sugar (deoxyribose) and phosphate groups. Attached to each sugar is one of four bases--adenine (A), cytosine (C), guanine (G), and thymine (T).

There are two types of DNA in the cell – autosomal DNA and mitochondrial DNA. Autosomal DNA (also called nuclear DNA) is packaged into 22 paired chromosomes. In each pair of autosomes, one was inherited from the mother and one was inherited from the father.

There are 2 chromosomes.

The two strands are held together by hydrogen bonds between the bases, with adenine forming a base pair with thymine, and cytosine forming a base pair with guanine.

Human cells contain 23 pairs of chromosomes (46 chromosomes in total). Each chromosome is formed by 2 strands of DNA that hydrogen-bonded to each other make the classic DNA double helix (double-stranded DNA).

Which planet is the smallest and the closest to the Sun?
O Earth
O Mars
O Mercury
O Venus

Answers

Answer:

Mercury

Explanation:

Answer:

Mercury is the smallest and closest planet to the sun

Clouds form when the air is cooled below the _________.
relative humidity,
percent saturation,
water content,
dew point.

Answers

Answer:

dew point

Explanation:

you are so welcome

Why do scientists monitor the effect of each method

A- to stop humans from using wood as a resource

B-to prevent global temperatures from rising in the future

C- to study how ecosystems are different in different part of the world

D- to ensure that human have a sustainable supply of wood for the future

Answers

The answer is B since climate change is already a huge issue in today’s society

Which type of wavelength has the shortest wavelength ?

Answers

Gamma rays is the shortest
Answer: Gamma rays.

Radio waves, infrared rays, visible light, ultraviolet rays, X-rays, and gamma rays are all types of electromagnetic radiation. Radio waves have the longest wavelength, and gamma rays have the shortest

The cross Lpq/lPQ x lpq/lpq is carried out and the L gene is found to be in the middle. what would be the geneotype of the double crossover gametes in this cross?

Answers

Answer:

  PLQ (Double Recombinant gamete)   plq (Double Recombinant gamete)  

Explanation:

Cross:

Parentals) pLq/PlQ x plq/plq

Gametes) pLq (Parental)          plq (Parental)

                PlQ (Parental)

                pLQ (Recomb)

                plQ (Recomb)

                PLq (Recomb)

                Plq (Recomb)

                PLQ (Double Recom)

                plq (Double Recom)  

Parentals)

p----L-----qP.....l........Q

Simple recombinant)

Recombination occurs between p and L, OR between l and q.

P----L-----qp......l.....Qp----L-----QP.....l........q

Double recombinant)

Recombination occurs between p and L, AND between l and q.

p-----l------qP......L.......Q

What is point source pollution?
In point source pollution, the pollutants come from a ______
, easily identifiable source.

Answers

the answer would be single

Answer:

What is point source pollution?

In point source pollution, the pollutants come from a

single, easily identifiable source.

Explanation:

plato answer

Help ASAP please!!!!!!!

Answers

Answer:

je n'arrive pas à faire ton travail, je suis desolé

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