describe 2 ways of salting out proteins ​

Answers

Answer 1

The phenomenon known as "salting-out" occurs at very high ionic strengths, when protein solubility declines as ionic strength rises. As a result, salting out may be used to segregate proteins according to how soluble they are in salt solutions.

Because large levels of sodium chloride disturb the bonds and structure of the active site, the rate of enzyme activity will gradually decrease as the concentration of sodium chloride rises. As a result, some of the active sites get denaturized and the starch loses its ability to attach to them. As more enzymes get denatured and eventually cease to function, enzyme activity will steadily wane.

Describe 2 Ways Of Salting Out Proteins

Related Questions

Predict the nature of the indicated
covalent bond.

Answers

non-polar

Explanation :

Hydrogen and carbon have similar electronegativity values, so the C—H bond is not normally considered a polar covalent bond.

A 2.49 g sample of aniline (C6H5NH2, molar mass = 93.13 g/mol) was combusted in a bomb calorimeter with a heat capacity of 4.25 kJ/°C. If the temperature rose from 29.5°C to 69.8°C, determine the value of ΔH°comb for aniline.

Answers

The value of delta H° for aniline = 6415 Kj/mol

calculation

Step 1: find heat

Q (heat)  =  C (specific heat capacity)  x ΔT (change in temperature)

C= 4.25 kj/c°

ΔT = 69.8-29.5  = 40.3 c°

Q= 4.25 kj/c  x  40.3 c = 171.28 kj

Step 2:  find the moles of aniline

moles  = mass/molar  mass

= 2.49 g/ 93.13 g/mol =0.0267 moles

Step 3:  find delta H

171.28 kj/0.0267 mol = 6415 kj/mol

since the reaction is  exothermic  delta H = 6415 Kj/mol

Answer:

-6.41x10^3

Explanation:

2.49 is 3 significant figures, so your answer should reflect that. Since heat is lost to the surroundings, the reaction is exothermic, and the answer should be negative.

If winds blow against a mountain from the south, then on which slope would you find the least precipitation?

Answers

The northern slope will have the least precipitation if winds blow against a mountain from the south.

What is Slope?

This is referred to as the degree of steepness on a surface such as rocky areas etc.

When wind blows on the southern part of a mountains, it gets stuck which is why the northern slope have the least precipitation.

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Given the following equation, how many grams of PbCO3 will dissolve when 2.50 L of 1.50 M H+ is added to 3.00 g of PbCO3?

Given
PbCO3(s) + 2H+(aq) = Pb^2(aq)+ +H2O (l)+ CO2(g)

Answers

Yes I did this and I would say yes, I got a 100%

What is the freezing point of an aqueous suger (C12H22O11) solution that boils at 110C°?

Answers

Sucrose doesn't show appreciable dissociation in water, so i≈1 in this case. by extension, −7.3°C is the approximate freezing point of this solution.

What is the freezing point?

The temperature at which a liquid solidifies.

Sucrose doesn't show appreciable dissociation in water, so i≈1 in this case. by extension, −7.3°C is the approximate freezing point of this solution.

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1. 50.0 g of an unknown metal was heated to 95.0 °C and added to 45.0 g of water at 25.0 °C
in a calorimeter. Once the system reached equilibrium the final temperature was 28.5 °C.
Calculate the specific heat capacity of the metal with correct units.
2. 60.0 g of nickel heated to 100.0 °C was added to 55.0 g of water at 25.0 °C inside a
calorimeter and the system was allowed to reach equilibrium. What would be the final
temperature of the metal and water? (specific heat of Ni = 0.44 J/g °C)
.
3. One challenge during calorimetry is to minimize the heat lost to the environment during
the experiment. Suggest how you would minimize the heat loss during the experiment (read
the steps of the experiment before you answer the question)

Answers

Answer

Q = mcΔT

Qwater = -Qmetal

(100 g)(4.18 J/g°C)(25°C - 20°C) = -(50 g)c(25°C - 90.0°C)

2090 J = (3250 g °C)c

c = 0.643 J/g °C

Which of the following is a correct statement regarding the free energy?
It is energy available to create bonds.
It always has the same value.
It is stored between atoms.
It is known as kinetic energy.

Answers

Answer:

A

Explanation:

A corn plant has been genetically engineered. It produces pollen that can make its own toxins. These toxins protect the corn plant against pests. What is a likely impact on the environment around the corn plant?

Bird and butterfly populations around the area may increase.
The concentration of pesticides in a nearby lake would increase.
Newer strains of pesticide-resistant pests may develop.
The carbon dioxide level would increase in the area.

Answers

Answer:

I think the answer is "newer strains of pesticide - resistant pests may develop" as I learnt in Biology, but I'm not sure as I don't know anything about corn plants.

Explanation:

Answer:

newer strains of pesticide

Explanation:

What is the primary purpose of a rate law?
Oto determine if a chemical reaction will be exothermic or endothermic
O to determine the amount of product that is formed from the concentration of reactants
O to show the relationship between the concentration of reactants and the reaction rate
O to show a relationship between the amounts of gas that can dissolve into a liquid

Answers

The rate law uses the molar concentrations of reactants to determine the reaction rate. Hence, option (C) is the correct option

What is rate law ?

A rate law shows how the rate of a chemical reaction depends on reactant concentration.

For a reaction such as aA → products, the rate law generally has the form

rate = k[A]ⁿ,

where,

k is a proportionality constant called the rate constantn is the order of the reaction with respect to A.

Typically, increased concentrations of reactants increases the speed of the reaction, because there are more molecules colliding and reacting with each other.

Hence , The rate law uses the molar concentrations of reactants to determine the reaction rate. Hence, option (C) is the correct option

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One donut contains 350 kcal. Convert this to calories and joules

Answers

350 Kcal is equals to 350000 calorie and 1464400 Joule.

How to calculate energy content in food ?

The equation for calculating the energy content of a food source via calorimetry is as follows: Energy (joules) = Mass of water (g) × 4.2 (J/gºC) × Temperature increase (ºC)

1 Kcal = 1000 calorie

Therefore,

35 Kcal = 350 x 1000

            = 350000 calorie

Now,

1 calorie = 4.184 joule

Therefore,

35000 calorie = 350000 x 4.184

                        = 1464400 joule

Hence, 350 Kcal is equals to 350000 calorie and 1464400 Joule.

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3-chloropropylamine what is?

Answers

Answer:

a chemical compound

Calculate the mass of pure sodium chloride will yield enough hydrogen chloride gas to tralize 25 cm² of 0.5 M potassium trioxo- bonate(IV) solution. NACI=58.5, HC1 = 36.5, K,CO, = 138)​

Answers

It equals k, Co =138

Which of the following is the electron configuration of an atom in the ground state?
(a) 1s2 2s1
(b) 1s2 2s2 2p5 3s2
(c) 1s2 2s1 2p6
(d) 1s1 2s2

Answers

Answer:

(a) 1s2 2s1

Explanation:

Electron configurations of atoms are in their ground state when the electrons completely fill each orbital before starting to fill the next orbital.

Understanding the notation

It's important to know how to read and interpret the notation.

For example, the first part of option (a) says "1s2"

The "1" means the first level or shellThe "s" means in an s-orbitalThe "2" means there are 2 electrons in that orbitalOther things to know about electron orbitals

It important to know which orbitals are in each shell:

In level 1, there is only an s-orbitalIn level 2, there is an s-orbital and a p-orbitalin level 3, there is an s-orbital, a p-orbital, and a d-orbital (things get a little tricky when the d-orbitals get involved, but this problem is checking on the basic concept -- not the higher level trickery)

So, it's also important to know how many electrons can be in each orbital in order to know if they are full or not.  The electrons should fill up these orbitals for each level, in this order:

s-orbitals can hold 2p-orbitals can hold 6d-orbitals can hold 10 (but again, that's beyond the scope of this problem)

Examining how the electrons are filling the orbitals

For option (a):

the 1s orbital is filled with 2, andthe 2s orbital has a single electron in it with no other orbitals involved.

This is in it's ground state.

For option (b):

the 1s orbital is filled with 2, the 2s orbital is filled with 2,the 2p orbital has 5 (short of a full 6), andthe 3s orbital has a single electron in it.

Because the 3s orbital has an electron, but the lower 2p before it isn't full.  This is NOT in it's ground state.

For option (c):

the 1s orbital is filled with 2, the 2s orbital has 1 (short of a full 2), andthe 2p orbital is filled with 6

Although the 2p orbital is full, since the 2s orbital before it was not yet full, this is NOT in it's ground state.

For option (d):

the 1s orbital has 1 (short of a full 2), andthe 2s orbital is filled with 2

Again, despite that the final orbital (in this case, the 2s orbital), is full, since the 1s orbital before it was not yet full, this is NOT in it's ground state.

the amount of gram - atoms in two gramm of sulfur is (NB: S=32amu)​

Answers

Answer:

3.76375e+22

Explanation:

(2/32)×(6.022 × 10^23)

Avogadro's number is the number of atoms or molecules in one mole of any substance. In other words, a mole is 6.02X1023 of some chemical unit. The size of the chemical unit does not change Avogadro’s number. An analogy can be made using eggs. You can buy small, medium, or large eggs. The size of the eggs doesn’t affect how many eggs are in one dozen. Similarly, the size of the representative particle doesn’t affect how many are in one mole. Describe your own analogy to show the relationship between moles and the size of representative particles.

Answers

A machine that is limited to making 10,000 sheets of paper, regardless of shape or size does not affect the amount of paper that the machine makes.


Hope this helped :)

What is the molarity of a solution made by dissolving 3.09 moles of NaCl in 1.50 L of solution?

Answers

Answer:

2.06M NaCl

Explanation:

Molarity is defined as a ratio of moles of the solute (the substance you're dissolving) to Liters of solution (the volume of the finished solution).  It uses the letter capital M as an abbreviation for the unit.

We're given values for both numbers (with the correct units already), so substitute, and calculate the number part:

[tex]\dfrac{n_{solute}}{V_{solution}}=\dfrac{3.09 [\text{mol NaCl}]}{1.50 [\text{L solution}]}=2.06\dfrac{[\text{mol NaCl}]}{[\text{L solution}]}=2.06[\text{M NaCl}][/tex]

What is the molar concentration of Zn2+ ions in a solution, if the electrode potential value is 59mV less than the standard electrode potential value at 298 K?

Answers

Molar concentration of Zn2+ions in a solution is 3.481 mol/lit

The electrode potential value is 59mV

Temperature=298k

What is electrode potential?

It is a force of galvanic cell. basically it is the difference between an electrolyte and electrode.

equation formed- Zn → Zn2+ + 2e

              from Nernst equation-

E=E cell - 0.059 log [Zn2+]

[zn2+]=3.481 mol/lit

hence, Molar concentration of Zn2+ions in a solution is 3.481 mol/lit

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What are the rows of the periodic table called?
A. Periods
B. Elements
C. Atoms
D. Groups

Answers

Answer:

i believe its called A. periods

how many moles of FeCl3 are produced when 3.0 mole.of Fe react eith excess Cl2 gas?

Answers

the answer is going to be about 3.66 moles

Which element has properties most like Mg?

Answers

Answer:

The elements which are in the group

What is the percent yield for the reaction below when
705.0 g SO2 and 80.0 g 0₂ produce 586.0 g SO3?
2SO2(g) + O2(g) → 2SO3(g)

Answers

The percent yield would be 68.3%

Percent yield

This is given as:

Percent yield = yield/theoretical yield x 100%

From the equation:

[tex]2SO_2(g) + O_2(g) -- > 2SO_3(g)[/tex]

The mole ratio of the reactants is 2;1

Mole of 705 g SO2 = 705/64 = 11.02 moles

Mole of 80 g O2 = 80/32 = 2.5 moles

O2 is limiting.

Mole ratio of O2 and SO3 = 1:2

Mole equivalent of SO3 = 2.5 x 2 = 5.0 moles

Mass of 5 moles SO3 = 5 x 80 = 400 grams

Percent yield = 400/586 = 68.3%

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Corrosion may be regarded as the destruction of metal by:
Electrochemical action
Hydroelectric action
Electromechanical action
All of the above

Answers

All of the above I think it might not be right

Which of the following would cause an increase in the magnetic force
between two magnets?

A. Decreasing the separation between the two magnets
B. Increasing the separation between the two magnets
C. Decreasing the amount of excess charge on the first magnet
D. Increasing the amount of excess charge on the first magnet

Answers

Decreasing the separation between the two magnets will result in increase in the magnetic force between two magnets.

What is a Magnet?

This is referred to a substance which produces magnetic field and result in the attraction and repulsion of certain types  of things.

Increase in the separation will reduce the magnetic force and vice versa which is why option A was chosen.

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Calculate the mass in grams of each of the following using dimensional analysis:

a. 6.02 x 1023 atoms of Mg
3.01 x 1023 formula units of CaCl2
12.4 x 1015 molecules of formaldehyde (CH2O)

Answers

The mass in grams of each of the following using dimensional analysis is:

6.02 x 10²³ atoms of Mg = 12g of Mg3.01 x 10²³ formula units of CaCl2 = 55.5g of CaCl212.4 x 10¹⁵ molecules of formaldehyde (CH2O) = 0.000000618g of formaldehyde

How to calculate mass?

The mass of a substance can be calculated in different ways depending on whether molecules or moles are given.

First, we need to convert atoms/formula units/molecules to moles as follows:

6.02 × 10²³ ÷ 6.02 × 10²³ = 1 mole of Mg3.01 x 10²³ ÷ 6.02 × 10²³ = 0.5 moles of CaCl212.4 x 10¹⁵ ÷ 6.02 × 10²³ = 2.06 × 10-⁸ moles of formaldehyde

Next, we convert each mole value to mass as follows:

1 mole of Mg = 1 × 12 = 12g of Mg0.5 moles of CaCl2 = 0.5 × 111 = 55.5g of CaCl22.06 × 10-⁸ moles of CH2O = 0.000000618g of formaldehyde

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In the reaction below, 1.000 × 103 g LiOH is combined with 8.80 × 102 g CO2. The reaction produces 3.25 × 102 g H2O in an experiment.

CO2 + 2LiOH → Li2CO3 + H2O
theoretical yield

Answers

Answer:

CO2

Explanation:

100% CORRECT ON EDGE

The mass of water produced when 1.000 × 10³ g of LiOH is combined with 8.80 × 10² g CO₂  is 3.25 × 10² g H₂O.

What is mass?

Mass is defined as a dimensionless number used to describe the mass of a particle or item. The kilogram is the International System's (SI) preferred unit of mass (kg). Atoms make up everyday matter. A majority of an atom's mass is contained in its nucleus.

It is a neutralization process when lithium hydroxide and carbon dioxide combine to form water and lithium carbonate.

The reaction is [tex]\rm CO_2 + 2LiOH \rightarrow Li_2CO_3 + H_2O[/tex]

Neutralization reaction is defined as an acid and base reaction that produces an ionic molecule and potentially water.

Thus, the mass of water produced when 1.000 × 10³ g of LiOH is combined with 8.80 × 10² g CO₂  is 3.25 × 10² g H₂O.

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At a certain temperature this reaction follows first-order kinetics with a rate constant of 0.086 s^-1:

2H3PO4(aq)=P2O5(aq)+3H2O(aq)

Suppose a vessel contains H3PO4 at a concentration of 1.27 M . Calculate how long it takes for the concentration of H3PO4 to decrease to 7.0% of its initial value. You may assume no other reaction is important.

Round your answer to 2 significant digits.

Please, need this ASAP

Answers

It takes 31 s for 1.27 M H₃PO₄ to decrease its concentration to 7.0% of its initial value following first-order kinetics.

What is first-order kinetics?

First-order kinetics occur when a constant proportion of a reactant disappears per unit time.

Let's consider the following first-order kinetics reaction with a rate constant k = 0.086 s⁻¹.

2 H₃PO₄(aq) = P₂O₅(aq) + 3 H₂O(aq)

Given the initial concentation is [H₃PO₄]₀ = 1.27 M, the concentration representing 7.0% of this value is:

[H₃PO₄] = 7.0% × 1.27 M = 0.089 M

We can calculate the time elapsed (t) using the following expression.

ln ([H₃PO₄]/[H₃PO₄]₀) = - k × t

ln (0.089 M/1.27 M) = - 0.086 s⁻¹ × t

t = 31 s

It takes 31 s for 1.27 M H₃PO₄ to decrease its concentration to 7.0% of its initial value following first-order kinetics.

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Magnesium reacts with sulfuric acid according to the fol-
lowing equation. How many moles of H₂ are produced by
the complete reaction of 230. mg of Mg with sulfuric acid?
Mg(s) + H₂SO (aq) →
MgSO₂(aq) + H₂(g)

Answers

Answer:

The moles of H2 is 230-two hundred and thirty

Question 18 of 32
What is a covalent bond?
OA. A bond in which molecules share electrons
OB. A bond in which atoms share electrons
C. A bond between ions of the same charge

Answers

Answer: A. A bond in which molecules share electrons

Describe the characteristics that identify a sample of matter as being a substance.

Answers

No matter how big or little a material is, its properties remain the same. Size, form, color, and mass are the qualities that distinguish a material from non-substances. All of these qualities, including size, shape, color, and mass, can be seen and measured. Some characteristics are physical, while others are chemical. Physical characteristics include mass, volume, density, and color.  Viscosity and solubility are examples of chemical qualities.

Which statement best explains why gneiss is composed of layers but there are no layers in granite?

Gneiss is a sedimentary rock and granite is a metamorphic rock.
Gneiss is a metamorphic rock and granite is a sedimentary rock.
Granite is formed due to the slow cooling of magma below Earth's surface and gneiss is formed due to metamorphosis.
Granite is formed due to the accumulation of sediments and gneiss is typically formed due to intense heat and pressure.

Answers

Gneiss is a sedimentary rock and granite is a metamorphic rock. That is option A.

What are rocks?

Rocks are geological hard materials that are made up of various types which include:

Sedimentary rocks: These are rocks that made up of various layers formed from sediments. Example is the gnesis.

Metamorphic rocks: These are rocks that are form from pre existing rocks that undergoes some transformation. Example is granite

Therefore, Gneiss is a sedimentary rock and granite is a metamorphic rock.

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Answer:

C

Granite is formed due to the slow cooling of magma below Earth's surface and gneiss is formed due to metamorphosis.

Explanation:

I took the test!

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