A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. What antibiotic-resistance phenotype(s) (characteristics) will be found on the plate

Answers

Answer 1

Answer: Tetracycline.

Explanation:

A plasmid is a small circular DNA molecule found in bacteria that is separate from the bacterial chromosome and replicates independently of it. They encode for certain genes that play a key role in antibiotic resistance. Restriction enzymes are endonucleases that catalyze the cleavage of phosphodiester bonds in different regions located within a DNA strand. PstI is an example of an endonuclease, it is a type II restriction enzyme produced by the microorganism Providencia stuartii that possesses a restriction target in double-stranded DNA dependent on an unmethylated, palindromic, asymmetric sequence, and in this example, it cuts the plasmid at a single site in the ampicillin-resistance gene. After that, the DNA is annealed with another fragment of DNA and this new recombinant molecule is used to transform E. coli cells. Transformation is a key step in DNA cloning because it occurs after restriction enzyme digestion and ligation treatments and transfers newly made plasmids into bacteria. So these bacterias will have a new fragment of DNA, which still has a tetracycline resistance gene but it no longer has the ampicillin resistance gene because it was disrupted by the restiction enzyme. Thereby, the antibiotic resistance phenotype is the tetracycline resistace.


Related Questions

Bumblebees show some ability to control their own body temperature. During cold weather, bumblebees have been observed warming their flight muscles by shivering. The bees are able to maintain a body temperature several degrees above that of the surrounding air. Regulation of their internal body temperature is an example of A. diffusion B. synthesis C. respiration D. Homeostasis

Answers

Answer:

D. Homeostasis

Explanation:

An endocrine system refers to a series of ductless glands and organs responsible for the production and secretion of hormones that are used by the body for the performance of various functions such as metabolism, controlling growth, reproduction, mood, sleep, etc. These hormones are secreted directly into the circulatory system (blood) and then transported to the organs and tissues in the body.

Basically, the endocrine system contributes significantly to the state of homeostasis in the body.

Homeostasis can be defined as a process through which a living organism maintains a steady or stable physical, internal and chemical environment that is ideal to enhance life and proper functionality.

Hence, regulation of the internal body temperature of the bumblebee is an example of homeostasis.

Answer:

Homeostasis

Explanation:

Wildfires in Crater Lake burned approximately 33,000 acres in 2011, leaving 25 square miles of charred
timber and bare ground. The five-week fire burned the loblolly pine trees and seeds for which Bastrop
State Park is famous. Park rangers are hoping that new plant growth on the bare soil cover will
eventually be established as pine trees; however, immediately following a wildfire, the first plants which
emerge will most likely be grasses as a stage in-
a primary succession
b. secondary succession
c. tertiary succession
dclimax community

Answers

Answer:

Secondary succession

Explanation:

state another hormone that influences the concentration of glucose in the blood apart from insulin and glucose​

Answers

Answer:

Amylin

Epinephrine

cortisol

growth hormone


HELP PLZ ASAP GIVING BRAINLIEST!!

Answers

Answer:

what do they mean when they say "spheres" ???

It could be the lithosphere (land) hydrosphere (water) and the atmosphere (air)

Explanation:

if the water molecules are evaporating then there's obviously water involved (hydrosphere)if the water started to evaporate from the ground then lithosphere is involved, too. and the water is rising into the atmosphere, also.

Which phase of cell division is shown?






anaphase II


telophase I


telophase II


prophase I of meiosis

Answers

Anaphase cause telephone the cells split and prophase is the first step

Answer:

Anaphase II

Explanation:

I took the test

where do I place echinodermata on the phylogenetic tree​

Answers

Answer:

QUESTION:

where do I place Echinodermata on the phylogenetic tree​?

ANSWER:

Echinoderms include starfish, sea urchins, sand dollars, and feather stars. Echinoderms are found in many different ocean environments, but most are found in reefs.

Explanation:

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The following schemes show the design of a peptide-based chemical probe and the proposed mechanism of its activity-based labeling of protein tyrosine phosphatase, an enzyme that is responsible for removing the phosphate from the tyrosine residue(s) of a peptide in a peptide-sequence specific manner. Based your understanding of the schemes and the relevant lecture material, identify the key structural components of this probe that are responsible for (a) binding, (b) covalently modifying, and (c) reporting, respectively.

Answers

Answer:

hello some vital information is missing attached below is the information

answer :

a) nucleophile and alquene carbon.

b) F^- and PO[tex]_{3}[/tex]^-  , Nu  and  C=O

c) fluorescence

Explanation:

The key structural components that are responsible for the following are

a)For  Binding : the key components responsible for binding are nucleophile and alquene carbon.

b) For Covalently modifying : The key components responsible for covalently Modifying are F^- and PO[tex]_{3}[/tex]^-  , Nu  and  C=O

c) For reporting : The key components responsible for reporting is fluorescence and this is because the as seen in the second structure  tyrosine fluorescence is lost

The two tubes found in vascular plants are the ?
a.stamen and pistil
b. xylem and phloem
C. stem and leaves
d. pollen and ovule

Answers

Answer:

a

Explanation:

What characteristics do animals and protists share?
A. The ability to make their own food. B. The ability to move around.
C. The ability to break down decaying matter D. Having multiple cells.

Answers

Answer:

B

Explanation:

They both move around

how would fossil fuel affect non terrestrial organisms?

Answers

Answer:

Explanation:

Drilling, extraction and burning of fossil fuels, endangers many desert biomes.  Some deserts are used as dumping grounds for radioactive and chemical waste. Increasing dry periods and lack of vegetation from climate change can lead to more dust storms which further affect plant and animal species.

Put the following in order of size, from smallest to largest
chromosome, gene; base pair

Answers

Answer:

The correct order of size - base pair < gene < chromosome.

Explanation:

Nitrogen base-pair attaches with the phosphate group and a sugar molecule to form a building block of DNA or nucleic acids. Nucleic acids make the gene is the physical and functional unit of hereditary that takes the information from parent cells to daughter cells.

Chromosomes are a structure that appears thread-like and makes the genome with different chromosomes. These structures are made up of wrapped DNA or genes around histone proteins.

what term refers to a situation where a single phenotypic character is determined by the additive effects of two or more genes?

Answers

Answer:

Explanation:

Polygenic inheritance refers to a situation where a single phenotype character is determined by the addictive of two or more genes.

hope it helps :)

3. At the playground, which one is an example of a wheel and axle?
A. merry-go-round B. seesaw
C. slide
D. swing
4. Why is wheel and axle a simple machine?
A. It is simple.
C. It has a wheel.
B. It is a machine.
D. It makes our work easier.
5. Which simple machine would stairs be an example?
A. inclined plane
B. lever C. pulley D. wedge
6. A stiff bar that moves about a fixed point is a
A. lever B. wheel and axle C. wedge
D. inclined plane
7. Which of the following is the use of inclined plane?
A. To deliver a person from high area to low area.
B. To deliver a person from low area to high area.
C. To lift an object easier by changing the direction of the effort force.
D. It lessens the effort exerted in transporting weight over a distance and height:
8. Which simple machine is made of rigid or strong bar that is free to move at a fixed point?
A. lever
B. pulley
D. wheel and axle
C. wedge
9. Seesaw, scissors, pliers are examples of which kind of
lever as illustrated below?
A. First-class lever C. Second-class lever
B. Third Class lever D. Fourth-class lever
10. Which simple is used by the man to move the barrel as shown in the picture below?
A. inclined plane
B. lever
C. screw
D. wedge​

Answers

Answer:

3.B

4.B

5.A

6.D

7.A

8.B

9.D

10.A

Which of the following is not an advantage of renewable energy?
a. Reduce pollution
b. reduce greenhouse gas emissions
c. Reduce biodiversity losses
d. Reduce profits for oil and gas companies

Answers

Answer:

I'd say that the answer is 'd. Reduced profits for oil and gas companies'.

Which planet has the most similarities as the earth?

Answers

Answer:

mars

Explanation:

because it has almost the same size according to my teacher

Answer: Mars

Explanation:

Examples of organelles

Answers

Answer:

Nucleus, mitochondria, cell membrane, ribosome, golgi body

Explanation:

A(n) A species is any species that is essential to the health of an ecosystem.​

Answers

Answer:

Keystone Species

Explanation:

A species on which other species in an ecosystem largely depend, such that if it were removed the ecosystem would change drastically.

Answer:

A species is any species that is essential to the health of an ecosystem? Keystone species

Explanation:

So Keystone species is your answer.

An organ that produces or releases a chemical is called a ___________. 20pts URGENT HELP FOR SCIENCE BRANLIEST

Answers

Answer:

gland?

Explanation:


a) varigated
leaves accumulates less Food than non-varigated leaves under similar condition​

Answers

Answer:

Due to less number of chlorophyll.

Explanation:

The variegated  leaves accumulates or produces less amount of food than non-variegated leaves under similar condition​ because variegated  leaves has number of chlorophyll as compared to non-variegated  leaves. Chlorophyll is a green pigment that is responsible for the photosynthesis in plants, without chlorophyll no photosynthesis occurs in the plants. The variegated  leaves has less number of chlorophyll as compared to non-variegated  leaves which leads to less production of food in variegated  leaves.

As a scientific concept, race Group of answer choices A. is very significant because it gives us a clearer understanding of human behavior than the concept of culture provides. B. is evidenced by the exclusive possession of particular types of genes. C. aids in explaining human diversity. D. is not very significant because it gives us little insight into human behavior. E. Both B and D

Answers

Answer:

D. is not very significant because it gives us little insight into human behavior.

Explanation:

Science can be defined as a branch of intellectual and practical study which systematically observe a body of fact in relation to the structure and behavior of non-living and living organisms (animals, plants and humans) in the natural world through experiments.

As a scientific concept, race is not very significant because it gives us little insight into human behavior.

This ultimately implies that, race is a scientific concept that holds no significant or absolute value since the nineteenth century.

Hence, the difference between humans shouldn't be described based on race according to the United Nations Educational, Scientific and Cultural Organization (UNESCO).

What happens to a cell when It receives the signal to begin apoptosis ?​

Answers

Answer:

QUESTION:

What happens to a cell when It receives the signal to begin apoptosis?​

ANSWER:

Apoptosis begins when the nucleus of the cell begins to shrink. After the shrinking, the plasma membrane blebs and folds around different organelles. The blebs continue to form and the organelles fragment and move away from one another.

Explanation:

Hope that this helps! :)

Have a great rest of your day/night!

How does the movement of muscles attached to the ribcage and diaphragm initiate expiration? (3 points)

a
The muscles relax, which which flattens the diaphragm and increases the volume of the chest cavity.
b
The muscles relax, which pulls the ribcage downward and decreases the volume of the chest cavity.
c
The lungs contract and push the air from the lungs.
d
The lungs relax and pull the air into the lungs.

Answers

Answer: b

The muscles relax, which pulls the ribcage downward and decreases the volume of the chest cavity.

Explanation:

Which product is typically made using softwood?

Answers

Answer:

Explanation:

The answer is furniture

In​ DNA, what base pairs with guanine?
adenine
cytosine
thymine
guanine

Answers

cytosine always pairs with guanine :)

6. a) In what way is the genetic code in all organisms the same? (1 point)

Answers

Answer:

they all come from the same ancestor, and all organisms have DNA made of the same molecules that form our genetic code. DNA in every organism has the bases: A,T,C,G.

Also, don't use this if it's still June 2021 and you're doing ap ex, we might have the same teacher.

All living organisms use the same genetic code. This shows that all organisms share a common evolutionary history.

What is a genetic code ?

The genetic code is the set of rules used by living cells to translate information encoded within genetic material into proteins. It is a sequence of three DNA or RNA nucleotides that corresponds with a specific amino acid or stop signal during protein synthesis.

The genetic code is universal. Living systems use nucleic acids and the same three-base codons to direct the synthesis of proteins from amino acids. For example, the mRNA codon UUU, codes for phenylalanine in all cells of all organisms.

To know more about genetic code here

https://brainly.com/question/14725012

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Different species of bumblebee compete for flower nectar in their ecosystem.

a. True
b. False

Answers

If there is limited of food then they will compete but if there is an equal amount of food they won’t compete. So the answer is true.
The answer is true!! :).....

How were the mountains surrounding the Canyon formed?​

Answers

Most canyons were formed by a process of long-time erosion from a plateau or table-land level. The cliffs form because harder rock strata that are resistant to erosion and weathering remain exposed on the valley walls

Gene mutations can be caused by many things. These mutations are biologically important because they



can be passed to the offspring if they occur in any cell of the body


can result in a new variety of gene combinations in the species


are always harmful and therefore help to eliminate weak traits


occur at a regular rate and therefore can be controlled

Answers

Answer:

Can result in a new variety of gene combinations in the species

Explanation:

The third option is blatantly untrue- gene mutations are sometimes harmful, usually mundane and do nothing, and sometimes extremely helpful.

The fourth option is also untrue- genetic mutation is completely random.

This leaves us with the first two-

The first option is true, but it's not why genetic mutations are important, it's simply how they are passed on.

The second option is the awnser because gene diversity is extremely important in populations, and 'variety of gene combinations' is just another way to say gene diversity.

The element carbon has multiple bonding patterns. Which bonding pattern cannot be formed by carbon?

A. CC
B. C-C-C
C.C.C.C
D. C=C=C

Answers

Answer:

D

Explanation:

Because there is only one carbon it says they are equal

A. CC and C. C.C.C

Carbon has four valence electrons, so it can achieve a full outer energy level by forming four covalent bonds.When it bonds only with hydrogen, it forms compounds called hydrocarbons. Carbon can form single, double, or triple covalent bonds with other carbon atoms.

A carbon atom can form the following bonds:

Four single bonds. One double and two single bonds. Two double bonds. One triple bond with one single bond.

In options, B and D there are single and double formation between carbon atoms. Thus these are correct bonding patterns.

Therefore, bonding pattern cannot be formed by carbon is option A and C.

Learn more:

brainly.com/question/1861117

Determine the direction of blood flow through the chambers, vessels and valves of the heart and lungs. Summarize your findings below beginning with oxygen-poor blood from the body entering the heart and ending with oxygen-rich blood leaving the heart to re-supply the body. 1 Vena cavae (vessels) 8 (vessels) 2 Right atrium (chamber) 9 (chamber) 3 (valve) 10 (valve) 4 (chamber) 11 (chamber) 5 (valve) 12 (valve) 6 (vessels) 13 (vessels) 7 Through the lungs (O2 picked up) 14 Through the body (O2 released)

Answers

Answer:

1 Vena cavae (vessels)

2 Right atrium (chamber)

3 Tricuspid valve (valve)

4 Right ventricule (chamber)

5 Pulmonary valve (valve)

6 Pulmonary artery (vessels)

7 Through the lungs (O2 picked up)

8 Pulmonary veins (vessels)

9 Left atrium (chamber)

10 Mitral valve (valve)

11 Left ventricle (chamber)

12 Aortic valve (valve)

13 Aorta (vessels)

14 Through the body (O2 released)

Explanation:

The oxygenated blood travels through the arteries distributing oxygen to the cells. Then the deoxygenated blood goes through the veins and to the heart and the lungs.

The deoxygenated blood enters the heart through the vena cavae. From there, it goes to the right atrium. The accumulation of blood in the right atrium opens the tricuspid valve, and the blood goes to the right ventricle. Between the right ventricle and the pulmonary vein, it is the pulmonary valve. The valve stops blood from going back to the ventricle once it is in the pulmonary artery. The pulmonary artery carries the blood to the pulmonary arterioles and these to the pulmonary capillaries, where the blood picks up oxygen and releases carbon dioxide. This process happens in the lungs.

Once that the blood has oxygen, it goes from the capillaries to the pulmonary venules. Then, to the pulmonary vein. The pulmonary vein brings the blood to the left atrium, then it goes through the mitral valve and flows to the left ventricle. Between the left ventricle and the aorta, it is the aortic valve. A valve that stops the blood from going back to the ventricle once it went to the aorta. From the aorta, the oxygenated blood goes to the rest of the body.

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