A binding protein binds to a ligand L with a Kd of 400 nM. What is the concentration of ligand when Y is (a) 0.25, (b) 0.6, (c) 0.95?

Answers: (a) 133.33nm (b) 600nm (c) 7,600nm

Answers above are correct. Please show all steps, really trying to understand.

Answers

Answer 1

Answer:

(a) 133.33nm

(b) 600nm

(c) 7,600nm

Explanation:

The concentration of Y can be determined by using the formula:

[tex]Y = \dfrac{[L]}{k_d+[L]}[/tex]

where;

[L] = concentration of the binding ligand.

kd = 400 nm

Thus:

When Y = 0.25; we get :

[tex]0.25 = \dfrac{[L]}{400+[L]}[/tex]

0.25 (400 + [L]) = [L]

100 + 0.25[L] = [L]

100 = [L] - 0.25 [L]

100 = 0.75 [L]

[L] = 100/0.75

[L] = 133.33 nm

At, Y = 0.6

[tex]0.6 = \dfrac{[L]}{400+[L]}[/tex]

0.6 (400 + [L]) = [L]

240 + 0.6[L] = [L]

240 = [L] - 0.6 [L]

240 = 0.4 [L]

[L] = 240/0.4

[L] = 600 nm

At, Y = 0.95

[tex]0.95 = \dfrac{[L]}{400+[L]}[/tex]

0.95 (400 + [L]) = [L]

380 + 0.95[L] = [L]

380 = [L] - 0.95 [L]

380 = 0.05 [L]

[L] = 380/0.05

[L] = 7600 nm


Related Questions

How many moles of carbondioxide are produced when 0.2mol of sodium carbonate react with excess hydrovhloric acid

Answers

Answer:

0.2 moles of CO₂  are produced

Explanation:

Given data:

Moles of CO₂ produced = ?

Moles of Na₂CO₃ react = 0.2 mol

Solution:

Chemical equation:

Na₂CO₃ + 2HCl       →     2NaCl + CO₂ + H₂O

Now we will compare the moles of CO₂ with Na₂CO₃ .

                   Na₂CO₃          :             CO₂

                       1                  :               1

                    0.2                :             0.2

Thus, 0.2 moles of CO₂  are produced.

The pH of a solution prepared by mixing 50.0 mL of 0.125 M KOH and 50.0 mL of 0.125 M HCl is ________.

Answers

Answer:

7

Explanation:

This question is a neutralization reaction.

50 ml of 0.125 M of KOH = (50 x 0.125) = 6.25 ml

In this same way, 50 ml of 0.125M of HCl = 50x0.125

= 6.25 ml

Then KOH is going to neutralise the HCl fully suvh that the pH of the medium is going to be 7

(H+) + (OH-) = H2O

Then Kw = [H=][OH-]

= [H+]² = 10^-14

Such that [H+] = 10^-7

The pH would be = -log [H+]

= -log[10^-7]

When inputted on a calculator this gives us 7

= 7

This is our answer

9. What are the advantages of using an indicator to inform pH measurements? What are the advantages of using a pH meter?

Answers

Answer:

The advantages of using an indicator to inform pH measurements:

It gives a mathematically result of the pH, in addition, it gives the precise pH of solvent, and it also gives an idea of the straight of the solution also.

Now, the advantage of using a pH meter:

It is a rapid method to characterize between acids, bases. However, this method does not show how strong acid or base actually are, plus it tends to gives a range of acidity or basicity not quite accurate as a result.

List the pros and cons of combustion.

Answers

Answer:

burny stuff

Explanation:

Answer:

Explanation:

Majority of fuels are comprised of hydrocarbons or compounds that contain carbon and hydrogen atoms. In a perfect setting, oxygen is supposed to convert all the hydrogen to water while the carbon is transformed to carbon dioxide.

Unfortunately, achieving a perfect combustion process is near impossible in reality. Fuels burn when they react with the amount of oxygen in the air. With insufficient air, the combustion doesn’t push through normally and ends up as an incomplete or partial combustion. Instead of just carbon dioxide, water, and nitrogen, an impartial combustion process produces harmful byproducts like partially burned hydrocarbons (VOCs) and Nitrogen oxides. A loss in fuel economy also occurs because not all of the fuel is properly processed to produce energy.

who wants these points

Answers

Answer:

GRACIAS

Explanation:

Answer:

Thnx

Explanation:

What is the pH value of distilled water?

Answers

Answer:

7

Explanation:

The ph level should be 7, but because it absorbs carbon dioxide from the atmosphere it’s actually slightly acidic with a pH of 5.8

hlo good evening everyone​

Answers

Answer:

hi

Explanation:

Hello good evening' but for me it's a good night

Explanation:

Anyway, thank you for the points have a great day!

Production of 6.5 grams of C2H2 requires consumption of how many grams of H2O

Answers

Answer:

C

Explanation:

Grams 3/4

Answer:

9g

Explanation:

number of moles × the moleculars mass

Of the following, which element has the highest first ionization energy?
Li
Cs
At
F​

Answers

There are free tutor on my website just got to freetutor.com
I think it’s Li but I don’t totally know

how many moles are present in 10.0g aspartame

Answers

Answer:

Here is the answer:

Explanation:

did i just say moles i’m sorry but heat kinda question is this

Regional Metamorphism occurs when great masses of rock are exposed to pressure. What does this typically form?

Answers

Answer:

Gneiss forms by regional metamorphism from both high temperature and pressure. Quartzite and marble are the most commonly used metamorphic rocks.

H2SO4(aq)+NaOH(s)> (neutralization of both acidic protons)

Answers

H2SO4(aq)+NaOH(s)----->NA2SO4 + H20

When acid and base reaction takes place, it will form salt formation takes place. I.e., neutralization reaction. H₂SO₄+2NaOH→ Na₂SO₄+ 2H₂O.

What is neutralization reaction?

A neutralization reaction can be defined as a chemical reaction in which an acid and base quantitatively react together to form a salt and water as products.

H₂SO₄ is an acid and NaOH is a base. Acid react with base to form salt and water. Salt will be neutral charge and net ionic charge is zero.

Neutralization takes place when, complete dissociation takes place. If any partial dissociation, it will form acidic salt or neutral salt.

Thus complete dissociation neutral reaction is

H₂SO₄+2NaOH→ Na₂SO₄+ 2H₂O.

To find more about acid and base, refer the link below:

brainly.com/question/3941445

#SPJ2

-1
1 point
Which is not a force that causes erosion and deposition?
carbonation
1
glaciers
waves
2
water
3

Answers

carbonation isn’t a force that causes such

How many grams are in 1.50 moles of KMnO4?

A. 165.06
B. 660.24
C. 178.41
D. 237.06
E. 484.29

Answers

Explanation:

mole=mass/Molar mass

mass= mole×molar mass

m= 1.5 × (39+55+64)

m= 1.5 × 158

m= 237g

There are 237.06 grams in 1.50 moles of KMnO4.

HOW TO CALCULATE MASS:

The mass of a substance can be calculated by multiplying the number of moles in the substance by its molar mass. That is;

mass of KMnO4 = no. of moles × molar mass of KMnO4

Molar mass of KMnO4 = 39 + 55 + 16(4)

= 158g/mol

mass of KMnO4 = 158g/mol × 1.5mol

Mass of KMnO4 = 237g

Therefore, there are 237.06 grams in 1.50 moles of KMnO4.

Learn more at: https://brainly.com/question/15743584?referrer=searchResults

Networks can be made of bonded ____________________ or ____________________.

Answers

Bonded atoms or ions.

How many grams of water (H2O) are produced from 34 grams of oxygen (O2)? *

Answers

Answer:

Explanation:

complete combustion reaction of  ethane is given by the reaction

2C2H6+7O2..............4CO2+6H2O

no of moles in 34 grams of O2=34/32=1.063

7mole of O2 produced 6 moles of H2O

therefore 1.063 moles of O2 produced=1.063*6/7=0.9 moles

now 0.9 moles of H2O contain how much grams=0.9*18=16.2 grams

Which statement describes a way electromagnetic waves are different from
mechanical waves?
A. Electromagnetic waves can travel only through matter.
B. Electromagnetic waves can carry energy over a distance,
0 0
C. Electromagnetic waves can transfer matter through empty space.
D. Electromagnetic waves can travel through empty space,

Answers

Answer:

D. Electromagnetic waves can travel through empty space.

Explanation:

Answer:

its drum roll please D.) Electromagnetic waves can travel through empty space

Explanation:

please wash your hands and stay safe 2021

What are valence electrons? How would you determine the valence electrons of Oxygen?

Answers

Answer:

Valence electrons are the electrons located on the outer shell of the atom. To determine valence electrons of oxygen you can count how many electrons ae on the last shell of it's bohr diagram.

Explanation:

How many electrons are in the nucleus of an atom with an atomic number of
16?
O None. Electrons are not in the nucleus
08
O 16
O 32

Answers

Answer:

None. Electrons are not in the nucleus.

Explanation:

We know that an atom consist of electrons, protons and neutrons. Neutrons and protons are present inside the nucleus while electrons are present out side the nucleus. Electron has a negative charge and is written as e⁻. The mass of electron is 9.10938356×10⁻³¹ Kg . While mass of proton and neutron is 1.672623×10⁻²⁷Kg and 1.674929×10⁻²⁷ Kg respectively.

Symbol of proton= P⁺  

Symbol of neutron= n⁰  

The number of electron or number of protons are called atomic number while mass number of an atom is sum of protons and neutrons. The umber of protons and electrons are always equal to make the atom electrically neutral .

The atom having atomic number 16 have 16 electrons and these electrons are present out side the nucleus.

can someone help. posted this same question 3-4 times.
Alina wants to make some yummy sugar cookies. The recipe written in her book is: 2 3/4 cup flour + 1 cup butter + 1 1/2 cup sugar + 1 egg + 1 tsp baking soda + 1/2 tsp baking powder = 24 cookies But Alina has the following ingredients in her kitchen: 9 cups flour, 5 cups butter, 4.5 cups sugar, 8 eggs and a excess of baking soda and powder. 1. How many cookies can she make? 2. Which ingredient is her limiting reactant? 3. Besides, baking soda and powder, how many of each reactant will be left over in her kitchen?​

Answers

(JSYK: this is all considering that only whole batches are included and fractional batches aren't)

Answer: 3 batches (72 cookies), her limiting reactant should be sugar since she only has enough to make three batches exactly, and she will also have 3/4 a cup of flour left over, 2 cups butter, and 5 eggs, in addition to the excess of baking soda and baking powder.

Hope this helps, if not, comment below please!!!

A chemistry graduate student is given 250.mL of a 1.60M chlorous acid HClO2 solution. Chlorous acid is a weak acid with =Ka×1.1 x 10^2. What mass of NaClO2 should the student dissolve in the HClO2 solution to turn it into a buffer with pH =1.45?

Answers

Answer:

28.04g of NaClO2 must be dissolved

Explanation:

When NaClO2 and HClO2 are mixed in a solution, a buffer is produced. The pH of this buffer is obtained using H-H equation:

pH = pKa + log [NaClO2] / [HClO2]

Where pKa is -log Ka = 1.9586

And [NaClO2] and [HClO2] could be taken as moles of each compound.

Moles of HClO2 are:

250mL = 0.250L * (1.60mol / L) = 0.40 moles HClO2.

Replacing:

pH = pKa + log [NaClO2] / [HClO2]

1.45 = 1.9586 + log [NaClO2] / [0.40mol]

0.31mol = [NaClO2]

To convert these moles to mass we need to use molar mass of NaClO2: 90.44g/mol:

0.31mol * (90.44g / mol) =

28.04g of NaClO2 must be dissolved

The atomic number of beryllium (Be) is 4, and the atomic number of barium (Ba) is 56. Which comparison is best supported by this information?

a. They are in the same group because they have similar atomic masses, but they are in different periods because they have different chemical properties.

b. They are in the same group because they have similar chemical properties, but they are in different periods because they have very different atomic numbers.

c. They are in the same group because their atomic numbers are each multiples of four, but they are in different periods because they have different chemical properties.

d. They are in the same group because they have similar atomic masses, but they are in different periods because they have very different atomic numbers.

Answers

The answer is b) similar chemical properties but very different atomic numbers

Answer:

B: They are in the same group because they have similar chemical properties, but they are in different periods because they have very different atomic numbers.

Explanation:

I took the Unit test on EDG and I got it correct

Pls mark me brainliest

CH4+O2—-CO2+H2O what is the best classification for the unbalanced equations reaction and why

Answers

I don’t know what you mean by classification exactly but it is a redox equation. The reactant side of carbon is losing hydrogen to form carbon dioxide. And oxygen is gaining hydrogen which gives you the water. Redox reactions are also known as combustion reactions.

Who can do my chemistry? It's due tonight, please! It’s about solubility interactive

Answers

Answer:

I can, i love chemistry

Explanation:

the earth is 24,901 miles. how many hours would it take to travel around the earth if traveling 100 meters per second?

Answers

Answer:

24,901 x 1609.34 = 40074275

40074275/100 equals

400742.75 s

divided by 3600

111.31

Explanation:

What kinds of things can we learn by studying atoms?​

Answers

Answer:

how and why things react, like why something turns into a gas or why something explodes.

Explanation:

how many moles of nitroge are there in 50.0 g of nitrogen?

Answers

1.785714286 moles

The number of moles (n) for nitrogen is: [ n=50.0÷28.0 ] = 1.785714286 moles.

Which is an example of a effect of geotropism

Answers

Answer:

An example of geotropism is the movement of nutrients. minerals and water in a plant. This transport is accomplished by specialized parts of the plant, the xylem which is pronounced zylem and the phloem which is pronounced flowem are the straw like parts of a plant’s stem that move the stuff up and down.

Explanation:

Which structure could a scientist look for in a plant that would identify it as a club moss rather than a liverwort?
O phloem
O spores
O rhizoids
O flowers

Answers

the answer is rhizoids

Answer:

what e said

Explanation:

If the pressure on 36.0 mL of a gas at STP is changed to a pressure of 35.3 kPa at constant temperature, the new volume of the gas is:

Answers

The new volume of the gas : 103.334 ml

Further explanation

Boyle's Law  

At a constant temperature, the gas volume is inversely proportional to the pressure applied  

[tex]\rm p_1V_1=p_2.V_2\\\\\dfrac{p_1}{p_2}=\dfrac{V_2}{V_1}[/tex]

1 kPa = 0,00986923 atm

35.3 kPa = 0,3483839 atm

at STP ( 0°C, 1 atm) with volume 36 ml changed to 35.3 kPa :

P₁=1 atm

V₁=36 ml

P₂= 0,3483839 atm

The new volume(V₂) :

[tex]\tt V_2=\dfrac{P_1.V_1}{P_2}\\\\V_2=\dfrac{1\times 36}{0,3483839}=103.334~ml[/tex]

Other Questions
What were the two major effects of the invention of the printing press I NEED HELP PLEASE HELP ME Write an equation in slope-intercept form with the following information. Goes through the points (6, 2) and (7, 5).Slope= _____ y-intercept=_____ WIDAD 2Leccin 2Hay una fiesta maana y muchas personas van a ir. Escribe cmo ellos piensan arreglarseantes de salir de sus casas. (Write how each person plans to get ready.)afeitarsecepillarselavarsemaquillarsesecarse - to dryla carael pelolos dienteslas piernasel cuerpola toallael espejoel jabnel cepillola crema1. Celia2. Sergio y yo3. T4. Yo5. Cristbal y Eligionon tu amigo(a) cuando se levanta. Usa verbos The first state to ratify the new Constitution was:VirginiaNew YorkDelawareRhode Island HELP!!! An object accelerates from rest to 85 m/s over a distance of 36 m. What acceleration did itexperience? What holds amino acid chains within a protein in shape? PLEASE HELP how is communication relevant for addressing challenges in the world? provide an example (be specific) A contestant on the biggest loser lost 78.8 pounds over a period of two months. At the end of the two month period, the contestant weighed 194.3 pounds. How much did the contestant weigh when the show began? x/2-4/5+x/5+3x/10=1/5 Select the correct answerHow does the second paragraph refine the idea that it's important to protect historically significant architecture?OA It provides evidence that the architecture can be useful for new developers.OB It provides solutions for protecting historical architectureOC it provides examples of what the government has done to protect the architecture.OD it provides reasons that the architecture should be preserved. What are the coordinates of point A What type of substance do humans make? [tex]f(x) = 1 - 2x \\ g(x) = x + 4 \\ h(x) = {x}^{2} + 1[/tex][tex]hgf(x) = 4 {x}^{2} + px + q[/tex]Find the value of p and the value of q John has $80. Every week, he spends $7. Create an equation that represents how much money, m , he has after t weeks Read this passage about Amelia Earhart.Amelia Earhart was born July 24, 1897, in Kansas. She was an adventurous and fun-loving child. That adventurous spirit remained as she grew older. One day in 1920, after going on a plane ride, she was determined to fly planes. She worked at any job she could find to save up for flying lessons, which cost $1,000. At one point, she worked as a nurses aid and a social worker. Six months after receiving flying lessons, Amelia bought her own plane, and she flew as high as 14,000 feet, which set a world record for female pilots. A short time afterward, Amelia became the sixteenth female to earn a pilots license. In 1928, an opportunity arose for Amelia to participate in a transatlantic flight across the Atlantic Ocean as a copilot. This experience later came in handy when she became the first woman to fly solo across the Atlantic in 1938. She received many honors for this achievement, such as the Distinguished Flying Cross from Congress.As Amelia approached her fortieth birthday, she was planning her biggest trip yet: She wanted to be the first woman to fly around the world. Of course, she needed a lot of help to make this happen. She made a first attempt that failed and damaged her plane. But Earhart was not one to give up, so she made another attempt at the ground-breaking mission. She did make some progress by flying 7,000 miles to New Guinea but did not make it to her next stop, Howland Island. Sadly, Earharts plane disappeared, and, despite search and rescue attempts, she was never found. To this day, Earhart is known and celebrated for her bravery, persistence, and commitment to aviation.Which sentence from the passage contains information that could best be used to create a yearbook blurb about Amelia Earhart?As Amelia approached her fortieth birthday, she was planning her biggest trip yet. Sadly, Earharts plane disappeared, and, despite search and rescue attempts, she was never found. One day in 1920, after going on a plane ride, she was determined to fly planes.Six months after receiving flying lessons, Amelia bought her own plane, and she flew as high as 14,000 feet, which set a world record for female pilots. PLEASE TELL ME THE ANSWER NOW ? Plzzzzz HELPPP WITH DIS LOOK AT THE PHOTO A plant has 3 parts, parallel veins, no main tap root, and the endosperm is separate.Is this plant a monocot or a dicot? The temperature drops 2F every hour. Make a table and write an equation to represent the total temperature drop over time. How do I come out as nonbinary to my parents? I've already come out as pan, but coming out gender-wise is gonna be difficult. I'm not coming out just yet, but I will eventually, so I need tips.